Adenosine-activated potassium conductance in cultured striatal neurons

Laurence Trussell, M. B. Jackson

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

We have examined the effect of adenosine on the membrane properties of cultured embryonic mouse striatal neurons using patch electrode techniques. Adenosine at 50μM effectively blocked spontaneous action potential activity. Adenosine or 2-chloroadenosine caused a slow hyperpolarization of the membrane potential and, under voltage clamp, an outward current that was blocked by 1 mM theophylline. ATP also caused a hyperpolarization that was slower and weaker than the adenosine response and could be blocked by 1 mM theophylline. The current induced by adenosine appears to be carried by potassium since an inward current was generated by adenosine when the cells were internally perfused with cesium salts and the reversal potential of the outward current shifted 57 mV with a 10-fold change in extracellular potassium concentration. The adenosine response is voltage dependent in that the current evoked by adenosine is reduced at holding potentials more positive than -55 mV, despite a larger driving force. Though calcium influx is not required for adenosine to activate the potassium conductance, some components of the cytosol may be essential, since the response is lost during intracellular perfusion.

Original languageEnglish (US)
Pages (from-to)4857-4861
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number14
StatePublished - 1985
Externally publishedYes

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Corpus Striatum
Adenosine
Potassium
Neurons
Theophylline
2-Chloroadenosine
Cesium
Membrane Potentials
Cytosol
Action Potentials
Electrodes
Perfusion
Salts
Adenosine Triphosphate
Calcium
Membranes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Adenosine-activated potassium conductance in cultured striatal neurons. / Trussell, Laurence; Jackson, M. B.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 14, 1985, p. 4857-4861.

Research output: Contribution to journalArticle

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