Additives for vaccine storage to improve thermal stability of adenoviruses from hours to months

Maria Pelliccia, Patrizia Andreozzi, Jayson Paulose, Marco D'Alicarnasso, Valeria Cagno, Manuela Donalisio, Andrea Civra, Rebecca M. Broeckel, Nicole Haese, Paulo Jacob Silva, Randy P. Carney, Varpu Marjomäki, Daniel Streblow, David Lembo, Francesco Stellacci, Vincenzo Vitelli, Silke Krol

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Up to 80% of the cost of vaccination programmes is due to the cold chain problem (that is, keeping vaccines cold). Inexpensive, biocompatible additives to slow down the degradation of virus particles would address the problem. Here we propose and characterize additives that, already at very low concentrations, improve the storage time of adenovirus type 5. Anionic gold nanoparticles (10-8-10-6 M) or polyethylene glycol (PEG, molecular weight ∼8,000 Da, 10-7-10-4 M) increase the half-life of a green fluorescent protein expressing adenovirus from ∼48 h to 21 days at 37°C (from 7 to >30 days at room temperature). They replicate the known stabilizing effect of sucrose, but at several orders of magnitude lower concentrations. PEG and sucrose maintained immunogenicity in vivo for viruses stored for 10 days at 37°C. To achieve rational design of viral-vaccine stabilizers, our approach is aided by simplified quantitative models based on a single rate-limiting step.

Original languageEnglish (US)
Article number13520
JournalNature Communications
Volume7
DOIs
StatePublished - Nov 30 2016

Fingerprint

adenoviruses
vaccines
sucrose
viruses
Viruses
Adenoviridae
Polyethylene glycols
Sucrose
low concentrations
Thermodynamic stability
thermal stability
Vaccines
Hot Temperature
Viral Vaccines
Refrigeration
Green Fluorescent Proteins
half life
Gold
Virion
Nanoparticles

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Pelliccia, M., Andreozzi, P., Paulose, J., D'Alicarnasso, M., Cagno, V., Donalisio, M., ... Krol, S. (2016). Additives for vaccine storage to improve thermal stability of adenoviruses from hours to months. Nature Communications, 7, [13520]. https://doi.org/10.1038/ncomms13520

Additives for vaccine storage to improve thermal stability of adenoviruses from hours to months. / Pelliccia, Maria; Andreozzi, Patrizia; Paulose, Jayson; D'Alicarnasso, Marco; Cagno, Valeria; Donalisio, Manuela; Civra, Andrea; Broeckel, Rebecca M.; Haese, Nicole; Silva, Paulo Jacob; Carney, Randy P.; Marjomäki, Varpu; Streblow, Daniel; Lembo, David; Stellacci, Francesco; Vitelli, Vincenzo; Krol, Silke.

In: Nature Communications, Vol. 7, 13520, 30.11.2016.

Research output: Contribution to journalArticle

Pelliccia, M, Andreozzi, P, Paulose, J, D'Alicarnasso, M, Cagno, V, Donalisio, M, Civra, A, Broeckel, RM, Haese, N, Silva, PJ, Carney, RP, Marjomäki, V, Streblow, D, Lembo, D, Stellacci, F, Vitelli, V & Krol, S 2016, 'Additives for vaccine storage to improve thermal stability of adenoviruses from hours to months', Nature Communications, vol. 7, 13520. https://doi.org/10.1038/ncomms13520
Pelliccia M, Andreozzi P, Paulose J, D'Alicarnasso M, Cagno V, Donalisio M et al. Additives for vaccine storage to improve thermal stability of adenoviruses from hours to months. Nature Communications. 2016 Nov 30;7. 13520. https://doi.org/10.1038/ncomms13520
Pelliccia, Maria ; Andreozzi, Patrizia ; Paulose, Jayson ; D'Alicarnasso, Marco ; Cagno, Valeria ; Donalisio, Manuela ; Civra, Andrea ; Broeckel, Rebecca M. ; Haese, Nicole ; Silva, Paulo Jacob ; Carney, Randy P. ; Marjomäki, Varpu ; Streblow, Daniel ; Lembo, David ; Stellacci, Francesco ; Vitelli, Vincenzo ; Krol, Silke. / Additives for vaccine storage to improve thermal stability of adenoviruses from hours to months. In: Nature Communications. 2016 ; Vol. 7.
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