Acute and repeated systemic amphetamine administration

Effects on extracellular glutamate, aspartate, and serine levels in rat ventral tegmental area and nucleus accumbens

Chang Jiang Xue, Jeffrey P. Ng, Yong Li, Marina Wolf

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Recent work indicates an important role for excitatory amino acids in behavioral sensitization to amphetamine. We therefore examined, using in vivo microdialysis in awake rats, the effects of amphetamine on efflux of glutamate, aspartate, and serine in the ventral tegmental area and nucleus accumbens, brain regions important for the initiation and expression of amphetamine sensitization, respectively. Water-pretreated and amphetamine- pretreated rats were compared to determine if sensitization altered such effects. In both brain regions, Ca2+-dependent efflux of glutamate accounted for ~20% of basal glutamate efflux. A challenge injection of water or 2.5 mg/kg of amphetamine did not significantly alter glutamate, aspartate, or serine efflux in the ventral tegmental area or nucleus accumbens of water- or amphetamine-pretreated rats. However, 5 mg/kg of amphetamine produced a gradual increase in glutamate efflux in both regions that did not reverse, was observed in both water- and amphetamine-pretreated rats, and was prevented by haloperidol. Although increased glutamate efflux occurred with too great a delay to mediate acute behavioral responses to amphetamine, it is possible that repeated augmentation of glutamate efflux during repeated amphetamine administration results in compensatory changes in levels of excitatory amino acid receptors in the ventral tegmental area and nucleus accumbens that contribute to development or expression of amphetamine sensitization.

Original languageEnglish (US)
Pages (from-to)352-363
Number of pages12
JournalJournal of Neurochemistry
Volume67
Issue number1
StatePublished - Jul 1 1996
Externally publishedYes

Fingerprint

Tegmentum Mesencephali
Ventral Tegmental Area
Nucleus Accumbens
Amphetamine
Aspartic Acid
Serine
Rats
Glutamic Acid
Water
Brain
Excitatory Amino Acids
Microdialysis
Glutamate Receptors
Haloperidol

Keywords

  • Amphetamine
  • Glutamate
  • Microdialysis
  • Nucleus accumbens
  • Sensitization
  • Ventral tegmental area

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Acute and repeated systemic amphetamine administration : Effects on extracellular glutamate, aspartate, and serine levels in rat ventral tegmental area and nucleus accumbens. / Xue, Chang Jiang; Ng, Jeffrey P.; Li, Yong; Wolf, Marina.

In: Journal of Neurochemistry, Vol. 67, No. 1, 01.07.1996, p. 352-363.

Research output: Contribution to journalArticle

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