Activation of the mitochondrial permeability transition pore modulates Ca2+ responses to physiological stimuli in adult neurons

Anna Bell, Alexander Komarov, György Hajnóczky, Paolo Bernardi, Dennis Bourdette, Michael Forte

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The participation of mitochondria in cellular and neuronal Ca2+ homeostatic networks is now well accepted. Yet, critical tests of specific mitochondrial pathways in neuronal Ca2+ responses have been hampered because the identity of mitochondrial proteins that must be integrated within this dynamic system remain uncertain. One putative pathway for Ca2+ efflux from mitochondria exists through the formation of the permeability transition pore (PTP) that is often associated with cellular and neuronal death. Here, we have evaluated neuronal Ca2+ dynamics and the PTP in single adult neurons in wild-type mice and those missing cyclophilin D (CyPD), a key regulator of the PTP. Using high-resolution time-lapse imaging, we demonstrate that PTP opening only follows simultaneous activation with two physiological stimuli that generate critical threshold levels of cytosolic and mitochondrial Ca2+. Our results are the first to demonstrate CyPD-dependent PTP opening in normal neuronal Ca2+ homeostatic mechanisms not leading to activation of cell death pathways. As neurons in mice lacking CyPD are protected in a number of neurodegenerative disease models, the results suggest that improved viability of CyPD-knockout animals in these pathological states may be due to the transient, rather than persistent, activation of the PTP in mutant mitochondria, thereby shielding neurons from cytoplasmic Ca2+ overload.

Original languageEnglish (US)
Pages (from-to)831-842
Number of pages12
JournalEuropean Journal of Neuroscience
Volume33
Issue number5
DOIs
StatePublished - Mar 2011

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Permeability
Neurons
Mitochondria
Time-Lapse Imaging
Mitochondrial Proteins
Neurodegenerative Diseases
mitochondrial permeability transition pore
Cell Death
cyclophilin D

Keywords

  • Calcium homeostasis
  • Cortical neurons
  • Mitochondria
  • Mutant mice
  • Neurodegeneration neuroprotection

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Activation of the mitochondrial permeability transition pore modulates Ca2+ responses to physiological stimuli in adult neurons. / Bell, Anna; Komarov, Alexander; Hajnóczky, György; Bernardi, Paolo; Bourdette, Dennis; Forte, Michael.

In: European Journal of Neuroscience, Vol. 33, No. 5, 03.2011, p. 831-842.

Research output: Contribution to journalArticle

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