Abstract
Starfish oocytes were injected with mRNA for the serotonin 1c receptor or with rat brain poly A+ mRNA, incubated to allow expression of the membrane protein, then matured to eggs by addition of 1-methyladenine. Applying serotonin to these eggs caused cortical granule exocytosis like that occurring at fertilization. Because the serotonin 1c receptor specifically activates a G-protein, these results provide support for the hypothesis that sperm activate eggs by way of a receptor-G-protein interaction. The starfish oocyte may be a generally useful system for expression of exogenous mRNA for membrane proteins.
Original language | English (US) |
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Pages (from-to) | 465-469 |
Number of pages | 5 |
Journal | Molecular biology of the cell |
Volume | 1 |
Issue number | 6 |
DOIs | |
State | Published - May 1990 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology