Activation by serotonin of starfish eggs expressing the rat serotonin 1c receptor.

F. Shilling, Gail Mandel, L. A. Jaffe

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Starfish oocytes were injected with mRNA for the serotonin 1c receptor or with rat brain poly A+ mRNA, incubated to allow expression of the membrane protein, then matured to eggs by addition of 1-methyladenine. Applying serotonin to these eggs caused cortical granule exocytosis like that occurring at fertilization. Because the serotonin 1c receptor specifically activates a G-protein, these results provide support for the hypothesis that sperm activate eggs by way of a receptor-G-protein interaction. The starfish oocyte may be a generally useful system for expression of exogenous mRNA for membrane proteins.

Original languageEnglish (US)
Pages (from-to)465-469
Number of pages5
JournalCell regulation
Volume1
Issue number6
StatePublished - May 1990
Externally publishedYes

Fingerprint

Starfish
Eggs
Serotonin
GTP-Binding Proteins
Messenger RNA
Oocytes
Membrane Proteins
Exocytosis
Fertilization
Spermatozoa
Brain
serotonin 1C receptor

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Activation by serotonin of starfish eggs expressing the rat serotonin 1c receptor. / Shilling, F.; Mandel, Gail; Jaffe, L. A.

In: Cell regulation, Vol. 1, No. 6, 05.1990, p. 465-469.

Research output: Contribution to journalArticle

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