Accelerated evolution of PAK3-and PIM1-like kinase gene families in the zebra finch, taeniopygia guttata

Lesheng Kong, Peter V. Lovell, Andreas Heger, Claudio Mello, Chris P. Ponting

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Genes encoding protein kinases tend to evolve slowly over evolutionary time, and only rarely do they appear as recent duplications in sequenced vertebrate genomes. Consequently, it was a surprise to find two families of kinase genes that have greatly and recently expanded in the zebra finch (Taeniopygia guttata) lineage. In contrast to other amniotic genomes (including chicken) that harbor only single copies of p21-activated serine/threonine kinase 3 (PAK3) and proviral integration site 1 (PIM1) genes, the zebra finch genome appeared at first to additionally contain 67 PAK3-like (PAK3L) and 51 PIM1-like (PIM1L) protein kinase genes. An exhaustive analysis of these gene models, however, revealed most to be incomplete, owing to the absence of terminal exons. After reprediction, 31 PAK3L genes and 10 PIM1L genes remain, and all but three are predicted, from the retention of functional sites and open reading frames, to be enzymatically active. PAK3L, but not PIM1L, gene sequences show evidence of recurrent episodes of positive selection, concentrated within structures spatially adjacent to N-and C-terminal protein regions that have been discarded from zebra finch PAK3L genes. At least seven zebra finch PAK3L genes were observed to be expressed in testis, whereas two sequences were found transcribed in the brain, one broadly including the song nuclei and the other in the ventricular zone and in cells resembling Bergmann's glia in the cerebellar Purkinje cell layer. Two PIM1L sequences were also observed to be expressed with broad distributions in the zebra finch brain, one in both the ventricular zone and the cerebellum and apparently associated with glial cells and the other showing neuronal cell expression and marked enrichment in midbrain/thalamic nuclei. These expression patterns do not correlate with zebra finch-specific features such as vocal learning. Nevertheless, our results show how ancient and conserved intracellular signaling molecules can be co-opted, following duplication, thereby resulting in lineage-specific functions, presumably affecting the zebra finch testis and brain.

Original languageEnglish (US)
Pages (from-to)1923-1934
Number of pages12
JournalMolecular Biology and Evolution
Volume27
Issue number8
DOIs
StatePublished - 2010

Fingerprint

p21-Activated Kinases
Finches
Taeniopygia guttata
Equidae
Protein-Serine-Threonine Kinases
threonine
serine
phosphotransferases (kinases)
Phosphotransferases
gene
Genes
genes
brain
Genome
genome
Neuroglia
protein kinases
Protein Kinases
Testis
testes

Keywords

  • gene family expansion
  • PAK3-like
  • PIM1-like
  • positive selection
  • serine/threonine kinases
  • zebra finch

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Medicine(all)

Cite this

Accelerated evolution of PAK3-and PIM1-like kinase gene families in the zebra finch, taeniopygia guttata. / Kong, Lesheng; Lovell, Peter V.; Heger, Andreas; Mello, Claudio; Ponting, Chris P.

In: Molecular Biology and Evolution, Vol. 27, No. 8, 2010, p. 1923-1934.

Research output: Contribution to journalArticle

Kong, Lesheng ; Lovell, Peter V. ; Heger, Andreas ; Mello, Claudio ; Ponting, Chris P. / Accelerated evolution of PAK3-and PIM1-like kinase gene families in the zebra finch, taeniopygia guttata. In: Molecular Biology and Evolution. 2010 ; Vol. 27, No. 8. pp. 1923-1934.
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