Absence of SPARC leads to impaired lens circulation

Teri Greiling, Brad Stone, John I. Clark

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

SPARC is a matricellular glycoprotein involved in regulation of extracellular matrix, growth factors, adhesion, and migration. SPARC-null mice have altered basement membranes and develop posterior sub-capsular cataracts with cell swelling and equatorial vacuoles. Exchange of fluid, nutrients, and waste products in the avascular lens is driven by a unique circulating ion current. In the absence of SPARC, increased circulation of fluid, ions, and small molecules led to increased fluorescein distribution in vivo, loss of resting membrane polarization, and altered distribution of small molecules. Microarray analysis of SPARC-null lenses showed changes in gene expression of ion channels and receptors, matrix and adhesion genes, cytoskeleton, immune response genes, and cell signaling molecules. Our results confirm the hypothesis that the regulation of SPARC on cell-capsular matrix interactions can increase the circulation of fluid and ions in the lens, and the phenotype in the SPARC-null mouse lens is the result of multiple intersecting functional pathways.

Original languageEnglish (US)
Pages (from-to)416-425
Number of pages10
JournalExperimental Eye Research
Volume89
Issue number3
DOIs
StatePublished - Sep 1 2009
Externally publishedYes

Fingerprint

Lenses
Ions
Waste Products
Microarray Analysis
Vacuoles
Cytoskeleton
Fluorescein
Ion Channels
Basement Membrane
Cataract
Genes
Extracellular Matrix
Intercellular Signaling Peptides and Proteins
Glycoproteins
Phenotype
Gene Expression
Food
Membranes

Keywords

  • capsule
  • cataract
  • circulation
  • electrophysiology
  • lens
  • matrix
  • microarray
  • SPARC

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Absence of SPARC leads to impaired lens circulation. / Greiling, Teri; Stone, Brad; Clark, John I.

In: Experimental Eye Research, Vol. 89, No. 3, 01.09.2009, p. 416-425.

Research output: Contribution to journalArticle

Greiling, Teri ; Stone, Brad ; Clark, John I. / Absence of SPARC leads to impaired lens circulation. In: Experimental Eye Research. 2009 ; Vol. 89, No. 3. pp. 416-425.
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