Absence of immunoreactive cytokines in supernatants of individual preimplantation human embryos

David Seifer, R. Romero, D. Berlinsky, R. V. Haning

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

PROBLEM: The purpose of this study was to determine if it was possible to use commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantitate specific cytokine production from individual embryos. METHODS: We examined the supernatants of individual two to six cell preimplantation human embryos using commerically available kits for IL-1 alpha, IL-1 beta, IL-6, and LIF. RESULTS: Cytokines were undetectable in all media samples analyzed. CONCLUSION: While it is possible that individual preimplantation embryos may produce cytokines, we believe these concentrations are below the detectable limits of the commercial assays used in this study.

Original languageEnglish (US)
Pages (from-to)105-107
Number of pages3
JournalAmerican Journal of Reproductive Immunology
Volume30
Issue number2-3
StatePublished - 1993
Externally publishedYes

Fingerprint

Blastocyst
Cytokines
Interleukin-1alpha
Interleukin-1beta
Interleukin-6
Embryonic Structures
Enzyme-Linked Immunosorbent Assay

Keywords

  • Cytokines
  • ELISA
  • human preimplantation embryos

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

Absence of immunoreactive cytokines in supernatants of individual preimplantation human embryos. / Seifer, David; Romero, R.; Berlinsky, D.; Haning, R. V.

In: American Journal of Reproductive Immunology, Vol. 30, No. 2-3, 1993, p. 105-107.

Research output: Contribution to journalArticle

Seifer, David ; Romero, R. ; Berlinsky, D. ; Haning, R. V. / Absence of immunoreactive cytokines in supernatants of individual preimplantation human embryos. In: American Journal of Reproductive Immunology. 1993 ; Vol. 30, No. 2-3. pp. 105-107.
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