Absence of immunoreactive cytokines in supernatants of individual preimplantation human embryos

D. B. Seifer, R. Romero, D. Berlinsky, R. V. Haning

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

PROBLEM: The purpose of this study was to determine if it was possible to use commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantitate specific cytokine production from individual embryos. METHODS: We examined the supernatants of individual two to six cell preimplantation human embryos using commerically available kits for IL-1 alpha, IL-1 beta, IL-6, and LIF. RESULTS: Cytokines were undetectable in all media samples analyzed. CONCLUSION: While it is possible that individual preimplantation embryos may produce cytokines, we believe these concentrations are below the detectable limits of the commercial assays used in this study.

Original languageEnglish (US)
Pages (from-to)105-107
Number of pages3
JournalAmerican Journal of Reproductive Immunology
Volume30
Issue number2-3
StatePublished - Dec 1 1993

Keywords

  • Cytokines
  • ELISA
  • human preimplantation embryos

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

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