A technical assessment of the utility of reverse phase protein arrays for the study of the functional proteome in non-microdissected human breast cancers

Bryan T. Hennessy, Yiling Lu, Ana Maria Gonzalez-Angulo, Mark S. Carey, Simen Myhre, Zhenlin Ju, Michael A. Davies, Wenbin Liu, Kevin Coombes, Funda Meric-Bernstam, Isabelle Bedrosian, Mollianne McGahren, Roshan Agarwal, Fan Zhang, Jens Overgaard, Jan Alsner, Richard M. Neve, Wen Lin Kuo, Joe Gray, Anne Lise Borresen-DaleGordon Mills

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Introduction: The lack of large panels of validated antibodies, tissue handling variability, and intratumoral heterogeneity potentially hamper comprehensive study of the functional proteome in non-microdissected solid tumors. The purpose of this study was to address these concerns and to demonstrate clinical utility for the functional analysis of proteins in non-microdissected breast tumors using reverse phase protein arrays (RPPA). Methods: Herein, 82 antibodies that recognize kinase and steroid signaling proteins and effectors were validated for RPPA. Intraslide and interslide coefficients of variability were

Original languageEnglish (US)
Pages (from-to)129-151
Number of pages23
JournalClinical Proteomics
Volume6
Issue number4
DOIs
StatePublished - Dec 2010
Externally publishedYes

Fingerprint

Protein Array Analysis
Proteome
Breast Neoplasms
Antibodies
Tumors
Proteins
Phosphotransferases
Steroids
Functional analysis
Neoplasms
Tissue

Keywords

  • Breast cancer
  • Functional proteome
  • Kinase signaling
  • RPPA
  • Steroid signaling

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Clinical Biochemistry

Cite this

A technical assessment of the utility of reverse phase protein arrays for the study of the functional proteome in non-microdissected human breast cancers. / Hennessy, Bryan T.; Lu, Yiling; Gonzalez-Angulo, Ana Maria; Carey, Mark S.; Myhre, Simen; Ju, Zhenlin; Davies, Michael A.; Liu, Wenbin; Coombes, Kevin; Meric-Bernstam, Funda; Bedrosian, Isabelle; McGahren, Mollianne; Agarwal, Roshan; Zhang, Fan; Overgaard, Jens; Alsner, Jan; Neve, Richard M.; Kuo, Wen Lin; Gray, Joe; Borresen-Dale, Anne Lise; Mills, Gordon.

In: Clinical Proteomics, Vol. 6, No. 4, 12.2010, p. 129-151.

Research output: Contribution to journalArticle

Hennessy, BT, Lu, Y, Gonzalez-Angulo, AM, Carey, MS, Myhre, S, Ju, Z, Davies, MA, Liu, W, Coombes, K, Meric-Bernstam, F, Bedrosian, I, McGahren, M, Agarwal, R, Zhang, F, Overgaard, J, Alsner, J, Neve, RM, Kuo, WL, Gray, J, Borresen-Dale, AL & Mills, G 2010, 'A technical assessment of the utility of reverse phase protein arrays for the study of the functional proteome in non-microdissected human breast cancers', Clinical Proteomics, vol. 6, no. 4, pp. 129-151. https://doi.org/10.1007/s12014-010-9055-y
Hennessy, Bryan T. ; Lu, Yiling ; Gonzalez-Angulo, Ana Maria ; Carey, Mark S. ; Myhre, Simen ; Ju, Zhenlin ; Davies, Michael A. ; Liu, Wenbin ; Coombes, Kevin ; Meric-Bernstam, Funda ; Bedrosian, Isabelle ; McGahren, Mollianne ; Agarwal, Roshan ; Zhang, Fan ; Overgaard, Jens ; Alsner, Jan ; Neve, Richard M. ; Kuo, Wen Lin ; Gray, Joe ; Borresen-Dale, Anne Lise ; Mills, Gordon. / A technical assessment of the utility of reverse phase protein arrays for the study of the functional proteome in non-microdissected human breast cancers. In: Clinical Proteomics. 2010 ; Vol. 6, No. 4. pp. 129-151.
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