A suite of bioassays to evaluate CREB inhibitors

Bingbing X. Li, Xiangshu Xiao

Research output: Chapter in Book/Report/Conference proceedingChapter


The cyclic-AMP response element binding protein (CREB) is an important nuclear transcription factor and has been shown to be overexpressed and/or over-activated in many different cancer types, suggesting that targeting CREB is a novel approach for developing cancer therapies. Our lab discovered the first cell-permeable small molecule inhibitor of CREB, from which we further developed a potent CREB inhibitor with in vivo anti-cancer activity. In this article, we detailed our biochemical and cell-based bioassays to assess different small molecule CREB inhibitors.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
StateAccepted/In press - Jan 1 2019

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • CREB
  • Inhibitor
  • Luciferase
  • Protein-protein interaction
  • Reporter
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Li, B. X., & Xiao, X. (Accepted/In press). A suite of bioassays to evaluate CREB inhibitors. In Methods in Enzymology (Methods in Enzymology). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2019.11.002