A rhesus macaque model of pulmonary nontuberculous mycobacterial disease

Kevin Winthrop, Andrea Rivera, Flora Engelmann, Sasha Rose, Anne Lewis, Jennifer Ku, Luiz Bermudez, Ilhem Messaoudi

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

In this study, we sought to develop a nonhuman primate model of pulmonary Mycobacterium avium complex (MAC) disease. Blood and bronchoalveolar lavage fluid were collected from three female rhesus macaques infected intrabronchially with escalating doses of M. avium subsp. hominissuis. Immunity was determined by measuring cytokine levels, lymphocyte proliferation, and antigen-specific responses. Disease progression was monitored clinically and microbiologically with serial thoracic radiographs, computed tomography scans, and quantitative mycobacterial cultures. The animal subjected to the highest inoculum showed evidence of chronic pulmonary MAC disease. Therefore, rhesus macaques could provide a robust model in which to investigate host-pathogen interactions during MAC infection.

Original languageEnglish (US)
Pages (from-to)170-176
Number of pages7
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume54
Issue number2
DOIs
StatePublished - Feb 1 2016

Keywords

  • Granuloma
  • Macaque
  • Mycobacterium avium
  • Nontuberculous mycobacteria
  • T cells

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

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