A repeated IMP-binding motif controls oskar mRNA translation and anchoring independently of Drosophila melanogaster IMP

Trent P. Munro, Sunjong Kwon, Bruce Schnapp, Daniel St Johnston

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Zip code-binding protein 1 (ZBP-1) and its Xenopus laevis homologue, Vg1 RNA and endoplasmic reticulum-associated protein (VERA)/Vg1 RNA-binding protein (RBP), bind repeated motifs in the 3′ untranslated regions (UTRs) of localized mRNAs. Although these motifs are required for RNA localization, the necessity of ZBP-1/VERA remains unresolved. We address the role of ZBP-1/VERA through analysis of the Drosophila melanogaster homologue insulin growth factor II mRNA-binding protein (IMP). Using systematic evolution of ligands by exponential enrichment, we identified the IMP-binding element (IBE) UUUAY, a motif that occurs 13 times in the oskar 3′UTR. IMP colocalizes with oskar mRNA at the oocyte posterior, and this depends on the IBEs. Furthermore, mutation of all, or subsets of, the IBEs prevents oskar mRNA translation and anchoring at the posterior. However, oocytes lacking IMP localize and translate oskar mRNA normally, illustrating that one cannot necessarily infer the function of an RBP from mutations in its binding sites. Thus, the translational activation of oskar mRNA must depend on the binding of another factor to the IBEs, and IMP may serve a different purpose, such as masking IBEs in RNAs where they occur by chance. Our findings establish a parallel requirement for IBEs in the regulation of localized maternal mRNAs in D. melanogaster and X. laevis.

Original languageEnglish (US)
Pages (from-to)577-588
Number of pages12
JournalJournal of Cell Biology
Volume172
Issue number4
DOIs
StatePublished - Feb 13 2006

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Amino Acid Motifs
Protein Biosynthesis
Drosophila melanogaster
Protein Binding
Carrier Proteins
Messenger RNA
Endoplasmic Reticulum
RNA
RNA-Binding Proteins
Xenopus laevis
Oocytes
SELEX Aptamer Technique
Stored Messenger RNA
Mutation
Nucleotide Motifs
Proteins
3' Untranslated Regions
Intercellular Signaling Peptides and Proteins
Binding Sites
Insulin

ASJC Scopus subject areas

  • Cell Biology

Cite this

A repeated IMP-binding motif controls oskar mRNA translation and anchoring independently of Drosophila melanogaster IMP. / Munro, Trent P.; Kwon, Sunjong; Schnapp, Bruce; St Johnston, Daniel.

In: Journal of Cell Biology, Vol. 172, No. 4, 13.02.2006, p. 577-588.

Research output: Contribution to journalArticle

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