TY - JOUR
T1 - A potential screening technique for neurotransmitters in the CNS
T2 - Model studies in the cat spinal cord
AU - Woodward, William R.
AU - Lindström, Sivert H.
N1 - Funding Information:
This researchw as supportedb y NIH grantsN S-07848a nd NS-02253a nd by USPHS PostdoctoraFle llowship1 F22 NS-03409-01(W RW) and by an NIH Fogarty InternationaFle llowshipT W 02084( SHL). We gratefullya cknowledgteh e technicaal ssistancoef Mrs. T. Bolino, Ms. S. Wilson, Ms. E. Livingston,M s. D. Cox, Mr. J. Gagliardi,M r. J. LaFratta,M r. M. LaFratta, Mr. W. Dragun and Ms. S. Mates. We are especiallyin debtedt o Drs. TorstenW ieseland Edward Kravitz for their supportt hroughoutth is work and their assistancien the preparatioonf this manuscript.
PY - 1977/11/25
Y1 - 1977/11/25
N2 - This paper describes a potential screening technique for neurotranmitters in the CNS. The method uses the injection of small volumes of high specific activity radioactive transmitter precursor substances into regions of physiologically identified neuronal cell bodies, and the later identification of the substances transported down axons to target tissues. Experiments were performed in motoneurons in the cat spinal cords to test the feasibility of the method. Tritiated choline, glutamate, tyramine and tryptophan were pressure-injected into the ventral horn using glass micropipettes that were adapted to allow similtaneous physiological recording and injection. Only tritiated acetylcholine, two unidentified choline metabolites and a small amount of choline were found in the motor axons. The acetylcholine migrated at a rate of greater than 24 mm/day and the movement was blocked by colchicine. The spread of isotope from the injection site was measured by a direct chemical method and by autoradiography, and it was found that isotope spread 1-2 mm from the injection site. One unexpected finding in the autoradiographs was that the motoneurons were selectively labelled following choline injections.
AB - This paper describes a potential screening technique for neurotranmitters in the CNS. The method uses the injection of small volumes of high specific activity radioactive transmitter precursor substances into regions of physiologically identified neuronal cell bodies, and the later identification of the substances transported down axons to target tissues. Experiments were performed in motoneurons in the cat spinal cords to test the feasibility of the method. Tritiated choline, glutamate, tyramine and tryptophan were pressure-injected into the ventral horn using glass micropipettes that were adapted to allow similtaneous physiological recording and injection. Only tritiated acetylcholine, two unidentified choline metabolites and a small amount of choline were found in the motor axons. The acetylcholine migrated at a rate of greater than 24 mm/day and the movement was blocked by colchicine. The spread of isotope from the injection site was measured by a direct chemical method and by autoradiography, and it was found that isotope spread 1-2 mm from the injection site. One unexpected finding in the autoradiographs was that the motoneurons were selectively labelled following choline injections.
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U2 - 10.1016/0006-8993(77)91011-3
DO - 10.1016/0006-8993(77)91011-3
M3 - Article
C2 - 72591
AN - SCOPUS:0017709589
SN - 0006-8993
VL - 137
SP - 37
EP - 52
JO - Brain research
JF - Brain research
IS - 1
ER -