A physical map of chromosome 20 established using fluorescence in situ hybridization and digital image analysis

Trond Stokke, Colin Collins, Wen Lin Kuo, David Kowbel, Farideh Shadravan, Minna Tanner, Anne Kallioniemi, Olli P. Kallioniemi, Daniel Pinkel, Larry Deaven, Joe Gray

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The physical locations of 46 cosmid clones and 21 P1 clones were determined along the chromosome 20 axis relative to the p terminus (FLpter) using fluorescence in situ hybridization (FISH) and digital image microscopy. The cosmid clones were selected from the chromosomally enriched library LA20NC01. Nine P1 clones were selected from a pooled DuPont genomic library using PCR with primer pairs selected to amplify genetically mapped sequence-tagged sites. This information was used to relate the physical map to the genetic map. Twelve P1 clones were selected from the same library using PCR primer pairs that amplified known genes. Two of these, E2F and BCLX, had not been mapped previously.

Original languageEnglish (US)
Pages (from-to)134-137
Number of pages4
JournalGenomics
Volume26
Issue number1
DOIs
StatePublished - Mar 1 1995
Externally publishedYes

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Chromosomes, Human, Pair 20
Fluorescence In Situ Hybridization
Clone Cells
Cosmids
Libraries
Sequence Tagged Sites
Polymerase Chain Reaction
Genomic Library
Microscopy
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

A physical map of chromosome 20 established using fluorescence in situ hybridization and digital image analysis. / Stokke, Trond; Collins, Colin; Kuo, Wen Lin; Kowbel, David; Shadravan, Farideh; Tanner, Minna; Kallioniemi, Anne; Kallioniemi, Olli P.; Pinkel, Daniel; Deaven, Larry; Gray, Joe.

In: Genomics, Vol. 26, No. 1, 01.03.1995, p. 134-137.

Research output: Contribution to journalArticle

Stokke, T, Collins, C, Kuo, WL, Kowbel, D, Shadravan, F, Tanner, M, Kallioniemi, A, Kallioniemi, OP, Pinkel, D, Deaven, L & Gray, J 1995, 'A physical map of chromosome 20 established using fluorescence in situ hybridization and digital image analysis', Genomics, vol. 26, no. 1, pp. 134-137. https://doi.org/10.1016/0888-7543(95)80092-Z
Stokke, Trond ; Collins, Colin ; Kuo, Wen Lin ; Kowbel, David ; Shadravan, Farideh ; Tanner, Minna ; Kallioniemi, Anne ; Kallioniemi, Olli P. ; Pinkel, Daniel ; Deaven, Larry ; Gray, Joe. / A physical map of chromosome 20 established using fluorescence in situ hybridization and digital image analysis. In: Genomics. 1995 ; Vol. 26, No. 1. pp. 134-137.
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