Bleomycin treatment of PM2 DNA results in fragmentation of the genome at several specific sites. Application of restriction endonuclease digestion followed by bleomycin treatment has provided the basis for constructing a physical map of bleomycin fragmentation sites. Eleven sites have been located on the physical map relative to Hpa II, Pst I, and HindIII cleavage sites. The fragmentation sites are not clustered in a particular region of the PM2 genome but 3 of the 11 sites do occur between the Hpa II and Pst I cleavage sites, a segment of DNA which comprises 14% of the PM2 DNA length.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology