A novel nerve growth factor-responsive element in the stromelysin-1 (transin) gene that is necessary and sufficient for gene expression in PC12 cells

S. DeSouza, J. Lochner, C. M. Machida, L. M. Matrisian, Gary Ciment

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Stromelysin-1 (ST-1) is an extracellular matrix metalloproteinase whose expression is transcriptionally regulated by nerve growth factor (NGF) in the PC12 rat pheochromocytoma cell line. In this paper, we define sequences in the proximal ST-1 promoter that contain a novel NGF-responsive element(s). We show that this cis-acting promoter element can bind nuclear proteins from both untreated and NGF-treated PC12 cells in a specific and saturable manner and is sufficient to confer NGF-inducibility to a heterologous promoter. At least a portion of this NGF-responsive element lies within a 12-base pair region between positions -241 and -229 of the ST-1 promoter and bears no sequence homology to other known transcriptional elements. In contrast to what has been reported for fibroblasts, an AP1 site centered around position -68 does not seem to be involved in the growth factor regulation of ST-1 in PC12 cells. These results suggest that the NGF regulation of ST-1 gene expression involves different promoter elements, and possibly different transcription factors, from that described for ST-1 induction by other growth factors.

Original languageEnglish (US)
Pages (from-to)9106-9114
Number of pages9
JournalJournal of Biological Chemistry
Volume270
Issue number16
DOIs
StatePublished - 1995

Fingerprint

Matrix Metalloproteinase 3
PC12 Cells
Nerve Growth Factor
Gene expression
Genes
Gene Expression
Intercellular Signaling Peptides and Proteins
Fibroblasts
Sequence Homology
Nuclear Proteins
Matrix Metalloproteinases
Base Pairing
Extracellular Matrix
Rats
Transcription Factors
Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

A novel nerve growth factor-responsive element in the stromelysin-1 (transin) gene that is necessary and sufficient for gene expression in PC12 cells. / DeSouza, S.; Lochner, J.; Machida, C. M.; Matrisian, L. M.; Ciment, Gary.

In: Journal of Biological Chemistry, Vol. 270, No. 16, 1995, p. 9106-9114.

Research output: Contribution to journalArticle

DeSouza, S. ; Lochner, J. ; Machida, C. M. ; Matrisian, L. M. ; Ciment, Gary. / A novel nerve growth factor-responsive element in the stromelysin-1 (transin) gene that is necessary and sufficient for gene expression in PC12 cells. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 16. pp. 9106-9114.
@article{52d00cf5b3cb4c658be61770f0ff1270,
title = "A novel nerve growth factor-responsive element in the stromelysin-1 (transin) gene that is necessary and sufficient for gene expression in PC12 cells",
abstract = "Stromelysin-1 (ST-1) is an extracellular matrix metalloproteinase whose expression is transcriptionally regulated by nerve growth factor (NGF) in the PC12 rat pheochromocytoma cell line. In this paper, we define sequences in the proximal ST-1 promoter that contain a novel NGF-responsive element(s). We show that this cis-acting promoter element can bind nuclear proteins from both untreated and NGF-treated PC12 cells in a specific and saturable manner and is sufficient to confer NGF-inducibility to a heterologous promoter. At least a portion of this NGF-responsive element lies within a 12-base pair region between positions -241 and -229 of the ST-1 promoter and bears no sequence homology to other known transcriptional elements. In contrast to what has been reported for fibroblasts, an AP1 site centered around position -68 does not seem to be involved in the growth factor regulation of ST-1 in PC12 cells. These results suggest that the NGF regulation of ST-1 gene expression involves different promoter elements, and possibly different transcription factors, from that described for ST-1 induction by other growth factors.",
author = "S. DeSouza and J. Lochner and Machida, {C. M.} and Matrisian, {L. M.} and Gary Ciment",
year = "1995",
doi = "10.1074/jbc.270.16.9106",
language = "English (US)",
volume = "270",
pages = "9106--9114",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "16",

}

TY - JOUR

T1 - A novel nerve growth factor-responsive element in the stromelysin-1 (transin) gene that is necessary and sufficient for gene expression in PC12 cells

AU - DeSouza, S.

AU - Lochner, J.

AU - Machida, C. M.

AU - Matrisian, L. M.

AU - Ciment, Gary

PY - 1995

Y1 - 1995

N2 - Stromelysin-1 (ST-1) is an extracellular matrix metalloproteinase whose expression is transcriptionally regulated by nerve growth factor (NGF) in the PC12 rat pheochromocytoma cell line. In this paper, we define sequences in the proximal ST-1 promoter that contain a novel NGF-responsive element(s). We show that this cis-acting promoter element can bind nuclear proteins from both untreated and NGF-treated PC12 cells in a specific and saturable manner and is sufficient to confer NGF-inducibility to a heterologous promoter. At least a portion of this NGF-responsive element lies within a 12-base pair region between positions -241 and -229 of the ST-1 promoter and bears no sequence homology to other known transcriptional elements. In contrast to what has been reported for fibroblasts, an AP1 site centered around position -68 does not seem to be involved in the growth factor regulation of ST-1 in PC12 cells. These results suggest that the NGF regulation of ST-1 gene expression involves different promoter elements, and possibly different transcription factors, from that described for ST-1 induction by other growth factors.

AB - Stromelysin-1 (ST-1) is an extracellular matrix metalloproteinase whose expression is transcriptionally regulated by nerve growth factor (NGF) in the PC12 rat pheochromocytoma cell line. In this paper, we define sequences in the proximal ST-1 promoter that contain a novel NGF-responsive element(s). We show that this cis-acting promoter element can bind nuclear proteins from both untreated and NGF-treated PC12 cells in a specific and saturable manner and is sufficient to confer NGF-inducibility to a heterologous promoter. At least a portion of this NGF-responsive element lies within a 12-base pair region between positions -241 and -229 of the ST-1 promoter and bears no sequence homology to other known transcriptional elements. In contrast to what has been reported for fibroblasts, an AP1 site centered around position -68 does not seem to be involved in the growth factor regulation of ST-1 in PC12 cells. These results suggest that the NGF regulation of ST-1 gene expression involves different promoter elements, and possibly different transcription factors, from that described for ST-1 induction by other growth factors.

UR - http://www.scopus.com/inward/record.url?scp=0028948154&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028948154&partnerID=8YFLogxK

U2 - 10.1074/jbc.270.16.9106

DO - 10.1074/jbc.270.16.9106

M3 - Article

C2 - 7721824

AN - SCOPUS:0028948154

VL - 270

SP - 9106

EP - 9114

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 16

ER -