A novel murine model of human renal cell carcinoma spinal metastasis

Lin Wang, Shayan Rahman, Chia Ying Lin, Juan Valdivia, Khoi Than, Frank La Marca, Paul Park

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

There is currently no reproducible animal model of renal cell carcinoma (RCC) spinal metastasis that allows for laboratory study of the human disease. In this report, we describe an animal model that reliably reproduces RCC spinal metastasis using a human tumor cell line. A posterior surgical approach was used to implant tumor cells into the lamina of immunosuppressed mice. Histology sections were analyzed 12 weeks after tumor cell implantation to quantify the location and extent of tumor growth. RCC xenografts grew in treated animals (8 mice) with a reproducible pattern of growth. After implantation, tumor growth occurred primarily in the antero-posterior dimension. At 8 weeks after tumor cell implantation, there was visible tumor growth in all treated mice. Histologic correlation at 12 weeks after tumor cell implantation confirmed tumor growth involving primarily the paraspinal region and lamina. Our investigation resulted in an orthotopic model of human RCC spinal metastasis. Ultimately this will allow testing of targeted therapies for RCC with spinal involvement. Furthermore, this model can be expanded to develop similar spinal metastasis models for other tumor cell lines.

Original languageEnglish (US)
Pages (from-to)881-883
Number of pages3
JournalJournal of Clinical Neuroscience
Volume19
Issue number6
DOIs
StatePublished - Jun 2012

Keywords

  • Murine model
  • Renal cell carcinoma
  • Spinal metastasis
  • Tumor

ASJC Scopus subject areas

  • Surgery
  • Neurology
  • Clinical Neurology
  • Physiology (medical)

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    Wang, L., Rahman, S., Lin, C. Y., Valdivia, J., Than, K., Marca, F. L., & Park, P. (2012). A novel murine model of human renal cell carcinoma spinal metastasis. Journal of Clinical Neuroscience, 19(6), 881-883. https://doi.org/10.1016/j.jocn.2011.10.019