A novel in vitro assay for mouse IgE

Tony J. Hall, Marvin B. Rittenberg

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A simple and sensitive assay for screening mouse IgE-producing hybridomas is described. Rat basophil leukemia (RBL) cells (either live or glutaraldehyde fixed) were sensitized by mouse monoclonal IgE and then rosetted with antigen-coated sheep red blood cells. The assay is established using a mouse monoclonal IgE anti-DNP and was able to detect as little as 5 ng/ml IgE. The rosette assay is also a useful technique for studying the binding of mouse IgG monoclonal (MC) antibodies to RBL cells.

Original languageEnglish (US)
Pages (from-to)145-154
Number of pages10
JournalJournal of Immunological Methods
Volume80
Issue number2
DOIs
StatePublished - Jun 25 1985

Keywords

  • IgE and IgG Fc receptors
  • hybridoma screening
  • murine IgE and IgG
  • rat basophil leukemia

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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