A new tool for the serodiagnosis of acute Plasmodium falciparum malaria in individuals with primary infection

Klaus Früh, H. M. Müller, H. Bujard, Andrea Crisanti

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

We have developed an ELISA which detects, with high specificity, antibodies against a major surface protein of P. falciparum merozoites which is a processing product of the precursor glycoprotein gp190. This assay can be used in the diagnosis of acute malaria in individuals with primary infection. Two partial sequences of gp190 were expressed in E. coli as β-galactosidase (β-Gal) fusion proteins. The same sequences fused to chloramphenicol acetyltransferase (CAT) or mouse dihydrofolate reductase (DHFR) react with high frequency when sera of acute malaria patients are analyzed in immunoblots. Antibodies from such sera crosslink, via their antigen binding sites, the β-Gal fusions to the corresponding CAT or DHFR fusions adsorbed to a solid phase as demonstrated by the captured β-Gal activity. The assay is highly specific, shows extremely low cut off values and should therefore be widely applicable.

Original languageEnglish (US)
Pages (from-to)25-32
Number of pages8
JournalJournal of Immunological Methods
Volume122
Issue number1
DOIs
StatePublished - Aug 15 1989

Keywords

  • ELISA
  • Fusion protein
  • Malaria
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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