@article{ede4af83b22845dabe8d929b44bfcfd9,
title = "A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins",
abstract = "The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging.",
author = "Gra{\v z}vydas Lukinavi{\v c}ius and Keitaro Umezawa and Nicolas Olivier and Alf Honigmann and Guoying Yang and Tilman Plass and Veronika Mueller and Luc Reymond and Corr{\^e}a, {Ivan R.} and Luo, {Zhen Ge} and Carsten Schultz and Lemke, {Edward A.} and Paul Heppenstall and Christian Eggeling and Suliana Manley and Kai Johnsson",
note = "Funding Information: This work was supported by the Swiss National Science Foundation, the Chemical National Centre of Competence in Research Biology, European Research Council grant no. 243016-PALMassembly and the {\'E}cole Polytechnique F{\'e}d{\'e}rale de Lausanne. G.L. was supported by a Federation of European Biochemical Societies long-term fellowship. K.U. was supported by a Grant-in-Aid for Postdoctoral Fellowships for Foreign Researchers Fellows. C.S. is supported by TRR83, and T.P. by the Fonds der Chemischen Industrie. E.A.L. acknowledges funding from the Emmy Noether program of the Deutsche Forschungsgemeinschaft. E.A.L. and C.S. also acknowledge funding from the SPP 1623 of the Deutsche Forschungsgemeinschaft. The authors thank A. Schena, B. Mollwitz and P. G{\"o}nczy for sharing reagents and cell lines, S. Hell and S. Jakobs (MPI G{\"o}ttingen) for excellent support, Tanja Gilat (Max-Planck Institut (MPI) G{\"o}ttingen) for preparation of the cells and A. Sch{\"o}nle (MPI G{\"o}ttingen) for support with the software ImSpector.",
year = "2013",
month = feb,
doi = "10.1038/nchem.1546",
language = "English (US)",
volume = "5",
pages = "132--139",
journal = "Nature Chemistry",
issn = "1755-4330",
publisher = "Nature Publishing Group",
number = "2",
}