A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins

Gražvydas Lukinavičius, Keitaro Umezawa, Nicolas Olivier, Alf Honigmann, Guoying Yang, Tilman Plass, Veronika Mueller, Luc Reymond, Ivan R. Corrêa, Zhen Ge Luo, Carsten Schultz, Edward A. Lemke, Paul Heppenstall, Christian Eggeling, Suliana Manley, Kai Johnsson

Research output: Contribution to journalArticle

367 Citations (Scopus)

Abstract

The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging.

Original languageEnglish (US)
Pages (from-to)132-139
Number of pages8
JournalNature Chemistry
Volume5
Issue number2
DOIs
StatePublished - Feb 2013
Externally publishedYes

Fingerprint

Fluorophores
Microscopic examination
Cells
Infrared radiation
Proteins
Rhodamines
Silicon
Fluorescent Dyes
Labeling
Luminance
Tissue
Imaging techniques
Wavelength

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Lukinavičius, G., Umezawa, K., Olivier, N., Honigmann, A., Yang, G., Plass, T., ... Johnsson, K. (2013). A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nature Chemistry, 5(2), 132-139. https://doi.org/10.1038/nchem.1546

A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. / Lukinavičius, Gražvydas; Umezawa, Keitaro; Olivier, Nicolas; Honigmann, Alf; Yang, Guoying; Plass, Tilman; Mueller, Veronika; Reymond, Luc; Corrêa, Ivan R.; Luo, Zhen Ge; Schultz, Carsten; Lemke, Edward A.; Heppenstall, Paul; Eggeling, Christian; Manley, Suliana; Johnsson, Kai.

In: Nature Chemistry, Vol. 5, No. 2, 02.2013, p. 132-139.

Research output: Contribution to journalArticle

Lukinavičius, G, Umezawa, K, Olivier, N, Honigmann, A, Yang, G, Plass, T, Mueller, V, Reymond, L, Corrêa, IR, Luo, ZG, Schultz, C, Lemke, EA, Heppenstall, P, Eggeling, C, Manley, S & Johnsson, K 2013, 'A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins', Nature Chemistry, vol. 5, no. 2, pp. 132-139. https://doi.org/10.1038/nchem.1546
Lukinavičius G, Umezawa K, Olivier N, Honigmann A, Yang G, Plass T et al. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nature Chemistry. 2013 Feb;5(2):132-139. https://doi.org/10.1038/nchem.1546
Lukinavičius, Gražvydas ; Umezawa, Keitaro ; Olivier, Nicolas ; Honigmann, Alf ; Yang, Guoying ; Plass, Tilman ; Mueller, Veronika ; Reymond, Luc ; Corrêa, Ivan R. ; Luo, Zhen Ge ; Schultz, Carsten ; Lemke, Edward A. ; Heppenstall, Paul ; Eggeling, Christian ; Manley, Suliana ; Johnsson, Kai. / A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. In: Nature Chemistry. 2013 ; Vol. 5, No. 2. pp. 132-139.
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