A mouse cytomegalovirus glycoprotein, gp34, forms a complex with folded class I MHC molecules in the ER which is not retained but is transported to the cell surface

Maurits F. Kleijnen, Johannes B. Huppa, Pero Lucin, Siddhartha Mukherjee, Helen Farrell, Ann E. Campbell, Ulrich H. Koszinowski, Ann B. Hill, Hidde L. Ploegh

Research output: Contribution to journalArticle

157 Scopus citations

Abstract

Murine cytomegalovirus (MCMV) interferes with antigen presentation by means of retaining major histocompatibility complex (MHC) class I molecules in the endoplasmic reticulum (ER). Here we identify and characterize an MCMV-encoded glycoprotein, gp34, which tightly associates with properly conformed MHC class I molecules in the ER. Gp34 is synthesized in large quantities during MCMV infection and it leaves the ER only in association with MHC class I complexes. Many but not all class I molecules are retained in the ER during the early phase of MCMV infection, and we observe an inverse correlation between amounts of gp34 synthesized during the course of infection and class I retention. An MCMV deletion mutant lacking several genes, including the gene encoding gp34, shows increased class I retention. Thus, MCMV gp34 may counteract class I retention, perhaps to decrease susceptibility of infected cells to recognition by natural killer cells.

Original languageEnglish (US)
Pages (from-to)685-694
Number of pages10
JournalEMBO Journal
Volume16
Issue number4
DOIs
StatePublished - Feb 17 1997

Keywords

  • Antigen presentation
  • ER retention
  • MHC class I
  • Murine cytomegalovirus
  • gp34

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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