A monoclonal antibody specific for the phosphorylated epitope of rhodopsin: Comparison with other anti-phosphoprotein antibodies

G. Adamus; Z. S. Zam; J. H. McDowell; G. P. Shaw; P. A. Hargrave

doi:10.1089/hyb.1988.7.237

A monoclonal antibody specific for the phosphorylated epitope of rhodopsin: Comparison with other anti-phosphoprotein antibodies

G. Adamus, Z. S. Zam, J. H. McDowell, G. P. Shaw, P. A. Hargrave

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Monoclonal antibody A11-82P has been prepared and shown to recognize the phosphorylated epitope of bovine rhodopsin. Antibody A11-82P is quite specific for phosphorylated rhodopsin; only the 200K neurofilament protein has been found to cross react, and this requires 10³ more protein for the same extent of binding as evaluated by competition ELISA. An immunocytochemical study of light-adapted retina showed strong staining of rod outer segments. Survey of a variety of rat tissues showed no specific staining with A11-82P, further demonstrating that this antibody is quite specific for phosphorylated rhodopsin. Two other antibodies were found to bind both phosphorylated rhodopsin and the 200K neurofilament protein: RT-97 and MAP 1B3. Both antibodies also recognized other phosphoproteins and appear to be less specific in their structural requirements for a phosphoprotein epitope than is A11-82P.

Original language	English (US)
Pages (from-to)	237-247
Number of pages	11
Journal	Hybridoma
Volume	7
Issue number	3
DOIs	https://doi.org/10.1089/hyb.1988.7.237
State	Published - 1988
Externally published	Yes

ASJC Scopus subject areas

Immunology
Genetics

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10.1089/hyb.1988.7.237

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title = "A monoclonal antibody specific for the phosphorylated epitope of rhodopsin: Comparison with other anti-phosphoprotein antibodies",

abstract = "Monoclonal antibody A11-82P has been prepared and shown to recognize the phosphorylated epitope of bovine rhodopsin. Antibody A11-82P is quite specific for phosphorylated rhodopsin; only the 200K neurofilament protein has been found to cross react, and this requires 103 more protein for the same extent of binding as evaluated by competition ELISA. An immunocytochemical study of light-adapted retina showed strong staining of rod outer segments. Survey of a variety of rat tissues showed no specific staining with A11-82P, further demonstrating that this antibody is quite specific for phosphorylated rhodopsin. Two other antibodies were found to bind both phosphorylated rhodopsin and the 200K neurofilament protein: RT-97 and MAP 1B3. Both antibodies also recognized other phosphoproteins and appear to be less specific in their structural requirements for a phosphoprotein epitope than is A11-82P.",

author = "G. Adamus and Zam, {Z. S.} and McDowell, {J. H.} and Shaw, {G. P.} and Hargrave, {P. A.}",

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T1 - A monoclonal antibody specific for the phosphorylated epitope of rhodopsin

T2 - Comparison with other anti-phosphoprotein antibodies

AU - Adamus, G.

AU - Zam, Z. S.

AU - McDowell, J. H.

AU - Shaw, G. P.

AU - Hargrave, P. A.

PY - 1988

Y1 - 1988

N2 - Monoclonal antibody A11-82P has been prepared and shown to recognize the phosphorylated epitope of bovine rhodopsin. Antibody A11-82P is quite specific for phosphorylated rhodopsin; only the 200K neurofilament protein has been found to cross react, and this requires 103 more protein for the same extent of binding as evaluated by competition ELISA. An immunocytochemical study of light-adapted retina showed strong staining of rod outer segments. Survey of a variety of rat tissues showed no specific staining with A11-82P, further demonstrating that this antibody is quite specific for phosphorylated rhodopsin. Two other antibodies were found to bind both phosphorylated rhodopsin and the 200K neurofilament protein: RT-97 and MAP 1B3. Both antibodies also recognized other phosphoproteins and appear to be less specific in their structural requirements for a phosphoprotein epitope than is A11-82P.

AB - Monoclonal antibody A11-82P has been prepared and shown to recognize the phosphorylated epitope of bovine rhodopsin. Antibody A11-82P is quite specific for phosphorylated rhodopsin; only the 200K neurofilament protein has been found to cross react, and this requires 103 more protein for the same extent of binding as evaluated by competition ELISA. An immunocytochemical study of light-adapted retina showed strong staining of rod outer segments. Survey of a variety of rat tissues showed no specific staining with A11-82P, further demonstrating that this antibody is quite specific for phosphorylated rhodopsin. Two other antibodies were found to bind both phosphorylated rhodopsin and the 200K neurofilament protein: RT-97 and MAP 1B3. Both antibodies also recognized other phosphoproteins and appear to be less specific in their structural requirements for a phosphoprotein epitope than is A11-82P.

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A monoclonal antibody specific for the phosphorylated epitope of rhodopsin: Comparison with other anti-phosphoprotein antibodies

Abstract

ASJC Scopus subject areas

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