A Moloney MLV-rat somatotropin fusion gene produces biologically active somatotropin in a transgenic pig

K. M. Ebert, M. J. Low, E. W. Overstrom, F. C. Buonomo, C. A. Baile, T. J. Roberts, A. Lee, Gail Mandel, Richard Goodman

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Expression of a Moloney murine leukemia virus (MLV) rat somatotropin fusion gene was examined in a transgenic pig. The fusion gene was integrated in a single site within the genome in a tandem array with approximately eight copies per cell. The integrated MLV-rat somatotropin fusion gene produced high levels of circulating rat somatotropin and resulted in an elevation in the circulating levels of insulin-like growth factor I. Although there was no increase in the rate of growth of the transgenic animal during the rapid growth phase, several phenotypic changes were evident. Skeletal growth was markedly increased and fat deposition was reduced throughout the animal. Blood glucose levels were elevated without ketosis. Northern blot analyses of rat somatotropin RNA revealed that expression of the fusion gene was highest in the spleen, lung, intestine, lymph nodes, and bone marrow. These results show that the MLV promoter can be used to express high levels of biologically active rat somatotropin in transgenic swine.

Original languageEnglish (US)
Pages (from-to)277-283
Number of pages7
JournalMolecular Endocrinology
Volume2
Issue number3
StatePublished - 1988
Externally publishedYes

Fingerprint

Moloney murine leukemia virus
Gene Fusion
Growth Hormone
Swine
Murine Leukemia Viruses
Growth
Ketosis
Genetically Modified Animals
Insulin-Like Growth Factor I
Northern Blotting
Intestines
Blood Glucose
Spleen
Lymph Nodes
Bone Marrow
Fats
Genome
RNA
Gene Expression
Lung

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Ebert, K. M., Low, M. J., Overstrom, E. W., Buonomo, F. C., Baile, C. A., Roberts, T. J., ... Goodman, R. (1988). A Moloney MLV-rat somatotropin fusion gene produces biologically active somatotropin in a transgenic pig. Molecular Endocrinology, 2(3), 277-283.

A Moloney MLV-rat somatotropin fusion gene produces biologically active somatotropin in a transgenic pig. / Ebert, K. M.; Low, M. J.; Overstrom, E. W.; Buonomo, F. C.; Baile, C. A.; Roberts, T. J.; Lee, A.; Mandel, Gail; Goodman, Richard.

In: Molecular Endocrinology, Vol. 2, No. 3, 1988, p. 277-283.

Research output: Contribution to journalArticle

Ebert, KM, Low, MJ, Overstrom, EW, Buonomo, FC, Baile, CA, Roberts, TJ, Lee, A, Mandel, G & Goodman, R 1988, 'A Moloney MLV-rat somatotropin fusion gene produces biologically active somatotropin in a transgenic pig', Molecular Endocrinology, vol. 2, no. 3, pp. 277-283.
Ebert KM, Low MJ, Overstrom EW, Buonomo FC, Baile CA, Roberts TJ et al. A Moloney MLV-rat somatotropin fusion gene produces biologically active somatotropin in a transgenic pig. Molecular Endocrinology. 1988;2(3):277-283.
Ebert, K. M. ; Low, M. J. ; Overstrom, E. W. ; Buonomo, F. C. ; Baile, C. A. ; Roberts, T. J. ; Lee, A. ; Mandel, Gail ; Goodman, Richard. / A Moloney MLV-rat somatotropin fusion gene produces biologically active somatotropin in a transgenic pig. In: Molecular Endocrinology. 1988 ; Vol. 2, No. 3. pp. 277-283.
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