A methylation-mediator complex in hormone signaling

Wei Xu, Helen Cho, Shilpa Kadam, Ester M. Banayo, Scott Anderson, John R. Yates, Beverly Emerson, Ronald M. Evans

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The recruitment of coactivators by nuclear hormone receptors (NRs) promotes transcription by subverting chromatin-mediated repression. Although the histone methylation enzyme CARM1 and an ATP-remodeling complex have been individually implicated in nuclear receptor-dependent transcription, neither a functional nor mechanistic linkage between these systems has been identified. In the process of purifying endogenous CARM1-interacting proteins, we identified an associated complex, nucleosomal methylation activator complex (NUMAC), which includes at least eight components of SWI/SNF, including the ATPase BRG1. In the NUMAC complex, the methylase, CARM1, acquires the ability to covalently modify nucleosomal histones, and the directed nucleosome versus free core histone methylation-specificity change is increased dramatically. Reciprocally, CARM1 stimulates the ATPase activity of BRG1, a key component in nucleosome remodeling. In vivo, CARM1 and BRG1 coassemble on an estrogen receptor (ER)-target gene to cooperatively activate ER-dependent transcription. This association of ATP-remodeling factors with HMT CARM1 defines a new component of regulation in the nuclear hormone-signaling pathway.

Original languageEnglish (US)
Pages (from-to)144-156
Number of pages13
JournalGenes and Development
Volume18
Issue number2
DOIs
StatePublished - Jan 15 2004
Externally publishedYes

Fingerprint

Mediator Complex
Methylation
Hormones
Histones
Nucleosomes
Cytoplasmic and Nuclear Receptors
Estrogen Receptors
Adenosine Triphosphatases
Adenosine Triphosphate
Chromatin
coactivator-associated arginine methyltransferase 1
Enzymes

Keywords

  • CARM1
  • Chromatin remodeling
  • Histone methyltransferase
  • SWI/SNF

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Xu, W., Cho, H., Kadam, S., Banayo, E. M., Anderson, S., Yates, J. R., ... Evans, R. M. (2004). A methylation-mediator complex in hormone signaling. Genes and Development, 18(2), 144-156. https://doi.org/10.1101/gad.1141704

A methylation-mediator complex in hormone signaling. / Xu, Wei; Cho, Helen; Kadam, Shilpa; Banayo, Ester M.; Anderson, Scott; Yates, John R.; Emerson, Beverly; Evans, Ronald M.

In: Genes and Development, Vol. 18, No. 2, 15.01.2004, p. 144-156.

Research output: Contribution to journalArticle

Xu, W, Cho, H, Kadam, S, Banayo, EM, Anderson, S, Yates, JR, Emerson, B & Evans, RM 2004, 'A methylation-mediator complex in hormone signaling', Genes and Development, vol. 18, no. 2, pp. 144-156. https://doi.org/10.1101/gad.1141704
Xu W, Cho H, Kadam S, Banayo EM, Anderson S, Yates JR et al. A methylation-mediator complex in hormone signaling. Genes and Development. 2004 Jan 15;18(2):144-156. https://doi.org/10.1101/gad.1141704
Xu, Wei ; Cho, Helen ; Kadam, Shilpa ; Banayo, Ester M. ; Anderson, Scott ; Yates, John R. ; Emerson, Beverly ; Evans, Ronald M. / A methylation-mediator complex in hormone signaling. In: Genes and Development. 2004 ; Vol. 18, No. 2. pp. 144-156.
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