A method for validating depth-resolved biodistributions in topically-stained specimen with multi-channel fluorescence cryo-imaging.

Brook K. Byrd, Rendall S. Strawbridge, Wendy Wells, Connor Barth, Summer Gibbs, Scott C. Davis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fluorescent contrast agents targeted to cancer biomarkers are increasingly being explored for cancer detection, surgical guidance, and response monitoring. Efforts have been underway to topically apply such biomarker-targeted agents to freshly excised specimen for detecting cancer cell receptors on the surface as a method for intraoperative surgical margin assessment, including dual-probe staining methods introduce a second ‘non-specific’ optical agent as a control to help compensate for heterogeneous uptake and normalize the imaging field. Still, such specimen staining protocols introduce multifaceted complexity with unknown variables, such as tissue-specific diffusion, cell-specific binding and disassociation rates, and other factors, affecting the interpreted cancer-biomarker distribution across the specimen surface. The ability to recover three-dimensional dual-probe biodistributions throughout whole-specimens could offer a ground-truth validation method for examining topical staining uptake behaviors. Herein, we report on a novel method for characterizing dual-probe accumulation with 3D depth-profiles observed from a dual-probe fresh-specimen staining experiment.

Original languageEnglish (US)
Title of host publicationMolecular-Guided Surgery
Subtitle of host publicationMolecules, Devices, and Applications VII
EditorsSylvain Gioux, Summer L. Gibbs, Brian W. Pogue
PublisherSPIE
ISBN (Electronic)9781510640856
DOIs
StatePublished - 2021
Externally publishedYes
EventMolecular-Guided Surgery: Molecules, Devices, and Applications VII 2021 - Virtual, Online, United States
Duration: Mar 6 2021Mar 11 2021

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11625
ISSN (Print)1605-7422

Conference

ConferenceMolecular-Guided Surgery: Molecules, Devices, and Applications VII 2021
Country/TerritoryUnited States
CityVirtual, Online
Period3/6/213/11/21

Keywords

  • Cryo-macrotome
  • Drug biodistribution
  • Molecular imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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