A method for quantification of calponin expression in myoepithelial cells in immunohistochemical images of ductal carcinoma in situ

Elliot Gray, Elizabeth Mitchell, Sonali Jindal, Pepper Schedin, Young Hwan Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Ductal carcinoma in situ (DCIS) is breast cancer confined within mammary ducts, surrounded by an intact myoepithelial cell layer that prevents local invasion. A DCIS diagnosis confers increased lifetime risk of developing invasive breast cancer (IBC) and results in surgical excision with radiation, and possibly endocrine- or chemo-therapy. DCIS is known to be over treated, with associated co-morbidities. Biomarkers are needed that delineate patients at low risk of DCIS progression from patients requiring more aggressive treatment. Investigating the role of myoepithelial cell differentiation in barrier function is anticipated to provide insight into DCIS progression and delineate between low and high risk lesions. Here, we develop a high throughput technique to assess loss of myoepithelial differentiation markers. This method facilitates automated analysis of a clinically relevant histopathologic feature, as demonstrated by a high correlation with pathologist annotation (r = 0.959), and further, contributes analytical foundations to a multiplexed immunohistochemistry (IHC) approach.

Original languageEnglish (US)
Title of host publication2018 IEEE 15th International Symposium on Biomedical Imaging, ISBI 2018
PublisherIEEE Computer Society
Pages796-799
Number of pages4
Volume2018-April
ISBN (Electronic)9781538636367
DOIs
StatePublished - May 23 2018
Event15th IEEE International Symposium on Biomedical Imaging, ISBI 2018 - Washington, United States
Duration: Apr 4 2018Apr 7 2018

Other

Other15th IEEE International Symposium on Biomedical Imaging, ISBI 2018
CountryUnited States
CityWashington
Period4/4/184/7/18

Fingerprint

Carcinoma, Intraductal, Noninfiltrating
Chemotherapy
Biomarkers
Ducts
Breast Neoplasms
Cells
Throughput
Differentiation Antigens
Radiation
Cell Differentiation
Breast
Immunohistochemistry
calponin
Morbidity
Therapeutics

Keywords

  • Calponin
  • DCIS
  • Feature engineering
  • Invasive breast cancer
  • Myoepithelial cell

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

Cite this

Gray, E., Mitchell, E., Jindal, S., Schedin, P., & Chang, Y. H. (2018). A method for quantification of calponin expression in myoepithelial cells in immunohistochemical images of ductal carcinoma in situ. In 2018 IEEE 15th International Symposium on Biomedical Imaging, ISBI 2018 (Vol. 2018-April, pp. 796-799). IEEE Computer Society. https://doi.org/10.1109/ISBI.2018.8363692

A method for quantification of calponin expression in myoepithelial cells in immunohistochemical images of ductal carcinoma in situ. / Gray, Elliot; Mitchell, Elizabeth; Jindal, Sonali; Schedin, Pepper; Chang, Young Hwan.

2018 IEEE 15th International Symposium on Biomedical Imaging, ISBI 2018. Vol. 2018-April IEEE Computer Society, 2018. p. 796-799.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Gray, E, Mitchell, E, Jindal, S, Schedin, P & Chang, YH 2018, A method for quantification of calponin expression in myoepithelial cells in immunohistochemical images of ductal carcinoma in situ. in 2018 IEEE 15th International Symposium on Biomedical Imaging, ISBI 2018. vol. 2018-April, IEEE Computer Society, pp. 796-799, 15th IEEE International Symposium on Biomedical Imaging, ISBI 2018, Washington, United States, 4/4/18. https://doi.org/10.1109/ISBI.2018.8363692
Gray E, Mitchell E, Jindal S, Schedin P, Chang YH. A method for quantification of calponin expression in myoepithelial cells in immunohistochemical images of ductal carcinoma in situ. In 2018 IEEE 15th International Symposium on Biomedical Imaging, ISBI 2018. Vol. 2018-April. IEEE Computer Society. 2018. p. 796-799 https://doi.org/10.1109/ISBI.2018.8363692
Gray, Elliot ; Mitchell, Elizabeth ; Jindal, Sonali ; Schedin, Pepper ; Chang, Young Hwan. / A method for quantification of calponin expression in myoepithelial cells in immunohistochemical images of ductal carcinoma in situ. 2018 IEEE 15th International Symposium on Biomedical Imaging, ISBI 2018. Vol. 2018-April IEEE Computer Society, 2018. pp. 796-799
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AB - Ductal carcinoma in situ (DCIS) is breast cancer confined within mammary ducts, surrounded by an intact myoepithelial cell layer that prevents local invasion. A DCIS diagnosis confers increased lifetime risk of developing invasive breast cancer (IBC) and results in surgical excision with radiation, and possibly endocrine- or chemo-therapy. DCIS is known to be over treated, with associated co-morbidities. Biomarkers are needed that delineate patients at low risk of DCIS progression from patients requiring more aggressive treatment. Investigating the role of myoepithelial cell differentiation in barrier function is anticipated to provide insight into DCIS progression and delineate between low and high risk lesions. Here, we develop a high throughput technique to assess loss of myoepithelial differentiation markers. This method facilitates automated analysis of a clinically relevant histopathologic feature, as demonstrated by a high correlation with pathologist annotation (r = 0.959), and further, contributes analytical foundations to a multiplexed immunohistochemistry (IHC) approach.

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