A lever-arm rotation drives motility of the minus-end-directed kinesin Ncd

Nicholas F. Endres, Craig Yoshioka, Ronald A. Milligan, Ronald D. Vale

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Kinesins are microtubule-based motor proteins that power intracellular transport1,2. Most kinesin motors, exemplified by Kinesin-1, move towards the microtubule plus end, and the structural changes that govern this directional preference have been described3-5. By contrast, the nature and timing of the structural changes underlying the minus-end-directed motility of Kinesin-14 motors (such as Drosophila Ncd6,7) are less well understood. Using cryo-electron microscopy, here we demonstrate that a coiled-coil mechanical element of microtubule-bound Ncd rotates ∼70° towards the minus end upon ATP binding. Extending or shortening this coiled coil increases or decreases velocity, respectively, without affecting ATPase activity. An unusual Ncd mutant that lacks directional preference8 shows unstable nucleotide-dependent conformations of its coiled coil, underscoring the role of this mechanical element in motility. These results show that the force-producing conformational change in Ncd occurs on ATP binding, as in other kinesins, but involves the swing of a lever-arm mechanical element similar to that described for myosins.

Original languageEnglish (US)
Pages (from-to)875-878
Number of pages4
JournalNature
Volume439
Issue number7078
DOIs
StatePublished - Feb 16 2006
Externally publishedYes

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Kinesin
Microtubules
Adenosine Triphosphate
Cryoelectron Microscopy
Myosins
Drosophila
Adenosine Triphosphatases
Nucleotides
Proteins

ASJC Scopus subject areas

  • General

Cite this

A lever-arm rotation drives motility of the minus-end-directed kinesin Ncd. / Endres, Nicholas F.; Yoshioka, Craig; Milligan, Ronald A.; Vale, Ronald D.

In: Nature, Vol. 439, No. 7078, 16.02.2006, p. 875-878.

Research output: Contribution to journalArticle

Endres, Nicholas F. ; Yoshioka, Craig ; Milligan, Ronald A. ; Vale, Ronald D. / A lever-arm rotation drives motility of the minus-end-directed kinesin Ncd. In: Nature. 2006 ; Vol. 439, No. 7078. pp. 875-878.
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