A human D1 dopamine receptor gene is located on chromosome 5 at q35.1 and identifies an EcoRI RFLP

D. K. Grandy, Q. Y. Zhou, L. Allen, R. Litt, R. E. Magenis, O. Civelli, M. Litt

    Research output: Contribution to journalArticle

    67 Scopus citations

    Abstract

    Dopaminergic neurons have been shown to affect voluntary movement, hormone secretion, and emotional tone. Mediating these activities are two receptor subtypes, D1 and D2, which are biochemically and pharmacologically distinct. The D1 subtype, the most abundant form of dopamine receptor in the central nervous system, stimulates adenylate cyclase, modulates D2 receptor activity, regulates neuron growth and differentiation, and mediates several behavioral responses. Recently we reported the cloning of a human D1 dopamine receptor gene (DRD1). High-stringency hybridization of the DRD1 clone to human genomic blots suggests that DRD1 is single copy. When used to probe a Southern blot made with DNAs from a rodent-human somatic cell hybrid panel, DRD1 hybridized to a 6.5-kb EcoRI restriction fragment which was assigned to chromosome 5. Fluorescent in situ hybridization of this gene to human metaphase chromosomes refined the location of DRD1 to 5q35.1. A search for RFLPs associated with DRD1 identified a two-allele EcoRI RFLP, allowing confirmation of DRD1's localization by linkage analysis in Centre d'Etude du Polymorphisme Humain families.

    Original languageEnglish (US)
    Pages (from-to)828-834
    Number of pages7
    JournalAmerican Journal of Human Genetics
    Volume47
    Issue number5
    StatePublished - 1990

    ASJC Scopus subject areas

    • Genetics
    • Genetics(clinical)

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    Grandy, D. K., Zhou, Q. Y., Allen, L., Litt, R., Magenis, R. E., Civelli, O., & Litt, M. (1990). A human D1 dopamine receptor gene is located on chromosome 5 at q35.1 and identifies an EcoRI RFLP. American Journal of Human Genetics, 47(5), 828-834.