A highly conserved RNA-binding protein for cytoplasmic mRNA localization in vertebrates

James O. Deshler, Martin I. Highett, Tatiana Abramson, Bruce Schnapp

Research output: Contribution to journalArticle

191 Citations (Scopus)

Abstract

Background: Cytoplasmic mRNA localization is a widespread mechanism for restricting the translation of specific mRNAs to distinct regions of eucaryotic cells. This process involves specific interactions between cellular factors and localization signals in the 3' untranslated regions of the localized mRNA. Because only a few of these cellular factors have been identified, it is not known whether common factors are utilized for the localization of different mRNAs. We recently discovered Vera, a protein that binds specifically to the Vg1 localization element and is involved in the localization of Vg1 mRNA in Xenopus oocytes. Results: To characterize further the role of Vera in the localization of Vg1 mRNA, we have purified the Vera protein and cloned its gene. Vera is homologous to chicken zip-code-binding protein (ZBP), which binds to a short RNA sequence required for localization of β-actin mRNA in chick embryo fibroblasts. The predicted amino-acid sequences of Vera and ZBP contain five RNA-binding domains and putative signals for nuclear localization and export. Like the binding of ZBP to β-actin mRNA, Vera specifically binds to a repeated sequence motif in the Vg1 localization element that is required for Vg1 mRNA localization in Xenopus oocytes. Conclusions: Vera, a highly conserved component of the mRNA localization machinery, participates in localizing different mRNAs in different cell types. Thus, Vera appears to be a general factor for mRNA localization, and additional factors may be required to specify diverse patterns of RNA localization.

Original languageEnglish (US)
Pages (from-to)489-496
Number of pages8
JournalCurrent Biology
Volume8
Issue number9
StatePublished - Apr 23 1998
Externally publishedYes

Fingerprint

RNA-binding proteins
RNA-Binding Proteins
Vertebrates
binding proteins
vertebrates
Xenopus
Messenger RNA
actin
oocytes
RNA
nuclear localization signals
3' untranslated regions
translation (genetics)
fibroblasts
eukaryotic cells
embryo (animal)
amino acid sequences
proteins
chicks
chickens

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

A highly conserved RNA-binding protein for cytoplasmic mRNA localization in vertebrates. / Deshler, James O.; Highett, Martin I.; Abramson, Tatiana; Schnapp, Bruce.

In: Current Biology, Vol. 8, No. 9, 23.04.1998, p. 489-496.

Research output: Contribution to journalArticle

Deshler, JO, Highett, MI, Abramson, T & Schnapp, B 1998, 'A highly conserved RNA-binding protein for cytoplasmic mRNA localization in vertebrates', Current Biology, vol. 8, no. 9, pp. 489-496.
Deshler, James O. ; Highett, Martin I. ; Abramson, Tatiana ; Schnapp, Bruce. / A highly conserved RNA-binding protein for cytoplasmic mRNA localization in vertebrates. In: Current Biology. 1998 ; Vol. 8, No. 9. pp. 489-496.
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