A fully integrated, three-dimensional fluorescence to electron microscopy correlative workflow

Claudia Lopez, Cedric Bouchet-Marquis, Christopher P. Arthur, Jessica McQuiston, Gregor Heiss, Guillaume Thibault, Lee Pullan, Sunjong Kwon, Joe Gray

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

While fluorescence microscopy provides tools for highly specific labeling and sensitive detection, its resolution limit and lack of general contrast has hindered studies of cellular structure and protein localization. Recent advances in correlative light and electron microscopy (CLEM), including the fully integrated CLEM workflow instrument, the FEI CorrSight with MAPS, have allowed for a more reliable, reproducible, and quicker approach to correlate three-dimensional time-lapse confocal fluorescence data, with three-dimensional focused ion beam-scanning electron microscopy data. Here we demonstrate the entire integrated CLEM workflow using fluorescently tagged MCF7 breast cancer cells.

Original languageEnglish (US)
JournalMethods in Cell Biology
DOIs
StateAccepted/In press - 2017

Fingerprint

Workflow
Electron Microscopy
Fluorescence
Light
Cellular Structures
Fluorescence Microscopy
Electron Scanning Microscopy
Ions
Breast Neoplasms
Proteins

Keywords

  • 3D FIB-SEM
  • CLEM
  • Correlative imaging technique
  • Electron microscopy
  • Fluorescence microscopy

ASJC Scopus subject areas

  • Cell Biology

Cite this

A fully integrated, three-dimensional fluorescence to electron microscopy correlative workflow. / Lopez, Claudia; Bouchet-Marquis, Cedric; Arthur, Christopher P.; McQuiston, Jessica; Heiss, Gregor; Thibault, Guillaume; Pullan, Lee; Kwon, Sunjong; Gray, Joe.

In: Methods in Cell Biology, 2017.

Research output: Contribution to journalArticle

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AU - Bouchet-Marquis, Cedric

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AU - Heiss, Gregor

AU - Thibault, Guillaume

AU - Pullan, Lee

AU - Kwon, Sunjong

AU - Gray, Joe

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