A fully integrated, three-dimensional fluorescence to electron microscopy correlative workflow

Claudia S. López, Cedric Bouchet-Marquis, Christopher P. Arthur, Jessica L. Riesterer, Gregor Heiss, Guillaume Thibault, Lee Pullan, Sunjong Kwon, Joe W. Gray

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

While fluorescence microscopy provides tools for highly specific labeling and sensitive detection, its resolution limit and lack of general contrast has hindered studies of cellular structure and protein localization. Recent advances in correlative light and electron microscopy (CLEM), including the fully integrated CLEM workflow instrument, the FEI CorrSight with MAPS, have allowed for a more reliable, reproducible, and quicker approach to correlate three-dimensional time-lapse confocal fluorescence data, with three-dimensional focused ion beam–scanning electron microscopy data. Here we demonstrate the entire integrated CLEM workflow using fluorescently tagged MCF7 breast cancer cells.

Original languageEnglish (US)
Pages (from-to)149-164
Number of pages16
JournalMethods in cell biology
Volume140
DOIs
StatePublished - 2017

Keywords

  • 3D FIB-SEM
  • CLEM
  • Correlative imaging technique
  • Electron microscopy
  • Fluorescence microscopy

ASJC Scopus subject areas

  • Cell Biology

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