A FlAsH-based cross-linker to study protein interactions in living cells

Anna Rutkowska, Christian H. Haering, Carsten Schultz

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

As you like it: xCrAsH, a dimeric derivative of the arsenical compound FlAsH, enables the highly specific, covalent cross-linking of two proteins containing a 12 amino acid peptide tag. This inducible and (by addition of dithiols) reversible system can be used to detect and manipulate protein-protein interactions both in vitro and in living cells (see picture).

Original languageEnglish (US)
Pages (from-to)12655-12658
Number of pages4
JournalAngewandte Chemie - International Edition
Volume50
Issue number52
DOIs
Publication statusPublished - Dec 23 2011
Externally publishedYes

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Keywords

  • biarsenical compounds
  • dimerization
  • protein cross-linking
  • protein-protein interactions
  • small molecules

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

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