A cell line selected for resistance to ionizing radiation exhibits cross resistance to other genotoxic agents and a mutator phenotype for loss of heterozygosity events

Kimberly A. Walker, C. Darrell Jennings, Joseph Pulliam, Charles Ogburn, George M. Martin, Muneyaso Urano, Mitchell Turker

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An ionizing radiation resistant derivative was obtained from the mouse P19H22 (aprt hemizygote) embryonal carcinoma cell line by repeated exposure to 137Cs gamma radiation. Ionizing radiation resistance in the 6Gy-R cell line was not correlated with a failure to undergo cell cycle arrest or a loss of the p53 response after exposure to 137Cs gamma radiation. Moreover, the cells did not display increased resistance to bleomycin, a double strand break inducing agent. However, the cells did display increased resistance to ultraviolet radiation, ethyl methanesulfonate, and 95% oxygen. A mutational analysis demonstrated a > 700fold-fold increase in the frequency of aprt mutants for the 6Gy-R cells, but no change in the frequency of hprt or dhfr mutants. A molecular analysis suggested that the aprt mutations in the 6Gy-R cells arose by recombinational events. A possible association between radiation resistance, DNA repair, and a mutator phenotype for large-scale mutational events is discussed.

Original languageEnglish (US)
Pages (from-to)111-121
Number of pages11
JournalSomatic Cell and Molecular Genetics
Volume23
Issue number2
StatePublished - Mar 1997

Fingerprint

Loss of Heterozygosity
Ionizing Radiation
Phenotype
Cell Line
Gamma Rays
Hemizygote
Radiation
Ethyl Methanesulfonate
Embryonal Carcinoma Stem Cells
Bleomycin
Cell Cycle Checkpoints
DNA Repair
Oxygen
Mutation

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

A cell line selected for resistance to ionizing radiation exhibits cross resistance to other genotoxic agents and a mutator phenotype for loss of heterozygosity events. / Walker, Kimberly A.; Jennings, C. Darrell; Pulliam, Joseph; Ogburn, Charles; Martin, George M.; Urano, Muneyaso; Turker, Mitchell.

In: Somatic Cell and Molecular Genetics, Vol. 23, No. 2, 03.1997, p. 111-121.

Research output: Contribution to journalArticle

Walker, Kimberly A. ; Jennings, C. Darrell ; Pulliam, Joseph ; Ogburn, Charles ; Martin, George M. ; Urano, Muneyaso ; Turker, Mitchell. / A cell line selected for resistance to ionizing radiation exhibits cross resistance to other genotoxic agents and a mutator phenotype for loss of heterozygosity events. In: Somatic Cell and Molecular Genetics. 1997 ; Vol. 23, No. 2. pp. 111-121.
@article{7e59b8c8d6544491b53b18c82624f756,
title = "A cell line selected for resistance to ionizing radiation exhibits cross resistance to other genotoxic agents and a mutator phenotype for loss of heterozygosity events",
abstract = "An ionizing radiation resistant derivative was obtained from the mouse P19H22 (aprt hemizygote) embryonal carcinoma cell line by repeated exposure to 137Cs gamma radiation. Ionizing radiation resistance in the 6Gy-R cell line was not correlated with a failure to undergo cell cycle arrest or a loss of the p53 response after exposure to 137Cs gamma radiation. Moreover, the cells did not display increased resistance to bleomycin, a double strand break inducing agent. However, the cells did display increased resistance to ultraviolet radiation, ethyl methanesulfonate, and 95{\%} oxygen. A mutational analysis demonstrated a > 700fold-fold increase in the frequency of aprt mutants for the 6Gy-R cells, but no change in the frequency of hprt or dhfr mutants. A molecular analysis suggested that the aprt mutations in the 6Gy-R cells arose by recombinational events. A possible association between radiation resistance, DNA repair, and a mutator phenotype for large-scale mutational events is discussed.",
author = "Walker, {Kimberly A.} and Jennings, {C. Darrell} and Joseph Pulliam and Charles Ogburn and Martin, {George M.} and Muneyaso Urano and Mitchell Turker",
year = "1997",
month = "3",
language = "English (US)",
volume = "23",
pages = "111--121",
journal = "Somatic Cell and Molecular Genetics",
issn = "0740-7750",
publisher = "Springer GmbH & Co, Auslieferungs-Gesellschaf",
number = "2",

}

TY - JOUR

T1 - A cell line selected for resistance to ionizing radiation exhibits cross resistance to other genotoxic agents and a mutator phenotype for loss of heterozygosity events

AU - Walker, Kimberly A.

AU - Jennings, C. Darrell

AU - Pulliam, Joseph

AU - Ogburn, Charles

AU - Martin, George M.

AU - Urano, Muneyaso

AU - Turker, Mitchell

PY - 1997/3

Y1 - 1997/3

N2 - An ionizing radiation resistant derivative was obtained from the mouse P19H22 (aprt hemizygote) embryonal carcinoma cell line by repeated exposure to 137Cs gamma radiation. Ionizing radiation resistance in the 6Gy-R cell line was not correlated with a failure to undergo cell cycle arrest or a loss of the p53 response after exposure to 137Cs gamma radiation. Moreover, the cells did not display increased resistance to bleomycin, a double strand break inducing agent. However, the cells did display increased resistance to ultraviolet radiation, ethyl methanesulfonate, and 95% oxygen. A mutational analysis demonstrated a > 700fold-fold increase in the frequency of aprt mutants for the 6Gy-R cells, but no change in the frequency of hprt or dhfr mutants. A molecular analysis suggested that the aprt mutations in the 6Gy-R cells arose by recombinational events. A possible association between radiation resistance, DNA repair, and a mutator phenotype for large-scale mutational events is discussed.

AB - An ionizing radiation resistant derivative was obtained from the mouse P19H22 (aprt hemizygote) embryonal carcinoma cell line by repeated exposure to 137Cs gamma radiation. Ionizing radiation resistance in the 6Gy-R cell line was not correlated with a failure to undergo cell cycle arrest or a loss of the p53 response after exposure to 137Cs gamma radiation. Moreover, the cells did not display increased resistance to bleomycin, a double strand break inducing agent. However, the cells did display increased resistance to ultraviolet radiation, ethyl methanesulfonate, and 95% oxygen. A mutational analysis demonstrated a > 700fold-fold increase in the frequency of aprt mutants for the 6Gy-R cells, but no change in the frequency of hprt or dhfr mutants. A molecular analysis suggested that the aprt mutations in the 6Gy-R cells arose by recombinational events. A possible association between radiation resistance, DNA repair, and a mutator phenotype for large-scale mutational events is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0030828892&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030828892&partnerID=8YFLogxK

M3 - Article

C2 - 9330639

AN - SCOPUS:0030828892

VL - 23

SP - 111

EP - 121

JO - Somatic Cell and Molecular Genetics

JF - Somatic Cell and Molecular Genetics

SN - 0740-7750

IS - 2

ER -