A Broccoli aptamer chimera yields a fluorescent K+sensor spanning physiological concentrations

Jonathan C. Savage, Pushkar Shinde, Yizhou Yao, Monika A. Davare, Ujwal Shinde

Research output: Contribution to journalArticlepeer-review

Abstract

The RNA aptamer Broccoli accepts 2′fluorinated (2′F) pyrimidine nucleotide incorporation without perturbation of structure or fluorescence in the presence of potassium and DFHBI. However, the modification decreases Broccoli's apparent affinity for K+>30-fold. A chimera of Broccoli RNAs with mixed chemistries displays linear fluorescent gain spanning physiological K+concentrations, yielding an effective RNA-based fluorescent K+sensor.

Original languageEnglish (US)
Pages (from-to)1344-1347
Number of pages4
JournalChemical Communications
Volume57
Issue number11
DOIs
StatePublished - Feb 7 2021

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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