Abstract
The RNA aptamer Broccoli accepts 2′fluorinated (2′F) pyrimidine nucleotide incorporation without perturbation of structure or fluorescence in the presence of potassium and DFHBI. However, the modification decreases Broccoli's apparent affinity for K+>30-fold. A chimera of Broccoli RNAs with mixed chemistries displays linear fluorescent gain spanning physiological K+concentrations, yielding an effective RNA-based fluorescent K+sensor.
Original language | English (US) |
---|---|
Pages (from-to) | 1344-1347 |
Number of pages | 4 |
Journal | Chemical Communications |
Volume | 57 |
Issue number | 11 |
DOIs | |
State | Published - Feb 7 2021 |
ASJC Scopus subject areas
- Catalysis
- Electronic, Optical and Magnetic Materials
- Ceramics and Composites
- General Chemistry
- Surfaces, Coatings and Films
- Metals and Alloys
- Materials Chemistry