6-Hydroxydopamine-mediated induction of rat brain metallothionein I mRNA

P. Rojas, D. R. Cerutis, H. K. Happe, L. C. Murrin, R. Hao, Ronald Pfeiffer, M. Ebadi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Oxidative stress, resulting either from excess generation or reduced scavenging of free radicals, has been proposed to play a role in damaging striatal neurons in Parkinson's disease. Since metallothionein is able to regulate the intracellular fedex potential, we have undertaken a group of experiments to learn whether or not 6-hydroxydopamine, which generates free radicals and is toxic to dopaminergic neurons, could alter the levels of zinc and metallothionein in the brain. The lesioning of the rat striatum with 6- hydroxydopamine (8.0μg in 4 μl 0.02% ascorbic acid) resulted in a reduction in the levels of zinc and metallothionein in the striatum but not other brain regions tested. However, the intracerebroventricular administration of 6- hydroxydopamine, in a dosage regimen that does not lesion catecholaminergic pathways but causes oxidative stress, enhanced dramatically the level of metallothionein I mRNA in some brain areas such as hippocampus, arcuate nucleus, choroid plexus, and granular layer of cerebellum, but not in the caudate putamen. The results of these studies are interpreted to suggest that zinc or metallothionein are altered in conditions where oxidative stress has taken place. Moreover, it is proposed that areas of brain, such as striatum containing high concentrations of iron, but low levels of inducible metallothionein are particularly vulnerable to oxidative stress.

Original languageEnglish (US)
Pages (from-to)323-334
Number of pages12
JournalNeuroToxicology
Volume17
Issue number2
StatePublished - 1996
Externally publishedYes

Fingerprint

Oxidative stress
Metallothionein
Oxidopamine
Rats
Brain
Oxidative Stress
Messenger RNA
Neurons
Free Radicals
Corpus Striatum
Arcuate Nucleus of Hypothalamus
Choroid Plexus
Poisons
Dopaminergic Neurons
Putamen
Scavenging
Cerebellum
Ascorbic Acid
Parkinson Disease
Zinc

Keywords

  • 6-hydroxydopamine
  • Free Radicals
  • Hippocampus
  • Metallothionein
  • Oxidative Stress
  • Striatum
  • Zinc

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neuroscience(all)
  • Toxicology

Cite this

Rojas, P., Cerutis, D. R., Happe, H. K., Murrin, L. C., Hao, R., Pfeiffer, R., & Ebadi, M. (1996). 6-Hydroxydopamine-mediated induction of rat brain metallothionein I mRNA. NeuroToxicology, 17(2), 323-334.

6-Hydroxydopamine-mediated induction of rat brain metallothionein I mRNA. / Rojas, P.; Cerutis, D. R.; Happe, H. K.; Murrin, L. C.; Hao, R.; Pfeiffer, Ronald; Ebadi, M.

In: NeuroToxicology, Vol. 17, No. 2, 1996, p. 323-334.

Research output: Contribution to journalArticle

Rojas, P, Cerutis, DR, Happe, HK, Murrin, LC, Hao, R, Pfeiffer, R & Ebadi, M 1996, '6-Hydroxydopamine-mediated induction of rat brain metallothionein I mRNA', NeuroToxicology, vol. 17, no. 2, pp. 323-334.
Rojas P, Cerutis DR, Happe HK, Murrin LC, Hao R, Pfeiffer R et al. 6-Hydroxydopamine-mediated induction of rat brain metallothionein I mRNA. NeuroToxicology. 1996;17(2):323-334.
Rojas, P. ; Cerutis, D. R. ; Happe, H. K. ; Murrin, L. C. ; Hao, R. ; Pfeiffer, Ronald ; Ebadi, M. / 6-Hydroxydopamine-mediated induction of rat brain metallothionein I mRNA. In: NeuroToxicology. 1996 ; Vol. 17, No. 2. pp. 323-334.
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