μ-Opioid receptor mRNA expression in identified hypothalamic neurons

Shi Xi Zheng, Martha A. Bosch, Oline Ronnekleiv

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

It has been known for a number of years that μ-opioid receptor agonists (e.g., morphine, β-endorphin, and enkephalin) inhibit luteinizing hormone (LH), vasopressin (VP), and oxytocin (OT) release and stimulate prolactin secretion in rodents and primates by an action at the level of the brain. Also, electrophysiological studies have established that hypothalamic neurons, including gonadotropin-releasing hormone (GnRH), VP, OT, β-endorphin, and dopamine neurons, are responsive to μ-receptor activation. Although μ-receptor expression has been demonstrated in the hypothalamus, there have been few studies localizing these receptors in neurosecretory neurons. Therefore, we sought to document μ-opioid receptor mRNA expression in immunocytochemically identified hypothalamic neurons. The brains from both female and male guinea pigs were examined by using in situ hybridization and immunocytochemistry. The studies revealed that μ-receptor mRNA was expressed in different diencephalic regions including the preoptic area, the bed nuclei stria terminalis, the paraventricular nucleus thalamus, and the anterior hypothalamus, as well as the supraoptic (SON), paraventricular (PVH), ventromedial, dorsomedial, and arcuate nuclei of the hypothalamus. Importantly, μ-opioid receptors were expressed in subpopulations of GnRH neurons (33.25 ± 4.6% and 33.6 ± 3.7% in females and males, respectively), dopamine neurons (51.7 ± 5.8% to 75.0 ± 2.6%, depending on neuronal location), β-endorphin neurons (68.3.0 ± 4.4%), and VP neurons (41-70%, depending on neuronal location). Because μ-opioid receptors couple via G-proteins to activate inwardly rectifying potassium channels and to inhibit calcium channels, the presence of these receptors is likely to play a major role in directly controlling the excitability of hypothalamic neurons.

Original languageEnglish (US)
Pages (from-to)332-344
Number of pages13
JournalJournal of Comparative Neurology
Volume487
Issue number3
DOIs
StatePublished - Jul 4 2005

Fingerprint

Opioid Receptors
Neurons
Messenger RNA
Endorphins
Vasopressins
Dopaminergic Neurons
Oxytocin
Gonadotropin-Releasing Hormone
Midline Thalamic Nuclei
Anterior Hypothalamic Nucleus
Inwardly Rectifying Potassium Channel
Mediodorsal Thalamic Nucleus
Septal Nuclei
Arcuate Nucleus of Hypothalamus
Preoptic Area
Enkephalins
Brain
Calcium Channels
Luteinizing Hormone
GTP-Binding Proteins

Keywords

  • Double labeling
  • Immunocytochemistry
  • In situ hybridization

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

μ-Opioid receptor mRNA expression in identified hypothalamic neurons. / Zheng, Shi Xi; Bosch, Martha A.; Ronnekleiv, Oline.

In: Journal of Comparative Neurology, Vol. 487, No. 3, 04.07.2005, p. 332-344.

Research output: Contribution to journalArticle

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