β1 adrenergic receptor and gαs mRNAs in rat heart as a function of mechanical load and thyroxine intoxication

Jean Marie Moalic, Francine Bourgeois, Pascale Mansier, Curtis Machida, François Carré, Brigitte Chevalier, Philippe Pitarque, Bernard Swynghedauw

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Objective: The aim of this study was to determine the expression of genes coding for the β1 adrenergic receptor and the α subunit of Gs in the adult rat normal and hypertrophied left ventricle, and in the left ventricle of the hypophysectomised rat after T4 intoxication. Methods: Total RNA was extracted from normal, control, or hypertrophied left ventricles 5 weeks after aortic stenosis, and from left ventricles of control or T4 injected hypophysectomised animals. The expression of β1 adrenergic receptor and Gαas mRNAs was quantitated by northern blot analysis and hybridisation with specific 32P-dCTP labelled DNA probes. Results: β1 Adrenergic receptor mRNA was decreased (by 33%) in compensated left ventricular hypertrophy without modification of the relative level of Gαs mRNA. The relative level of β1 adrenergic receptor mRNA correlated negatively with the degree of left ventricular hypertrophy, suggesting that the expression of the β1 adrenergic receptor gene is not activated by pressure overload. In the left ventricle of the hypophysectomised rat, a rapid increase in β1 adrenergic receptor mRNA (by 180% 3 h after hormone injection) was observed in response to T4, with no change in the relative content of Gαs mRNA. These results provide evidence that β1 adrenergic receptor mRNA and Gas mRNA accumulate to different levels of abundance in the adult left ventricle, as indicated by their ratios (0.053 and 0.043 in sham operated and hypertrophied left ventricles respectively). This suggests that distinct mechanisms are involved in the control of the accumulation of these two mRNAs in cardiac tissue. Conclusions: The reduction in β1 adrenergic receptor density in the hypertrophied rat left ventricle is associated with a parallel reduction in the level of β1 adrenergic receptor mRNA. The β1 adrenergic receptor gene may belong to a group of genes which are not activated by pressure overload, but are responsive to thyroid hormone.Cardiovascular Research 1993;27:231-237.

Original languageEnglish (US)
Pages (from-to)231-237
Number of pages7
JournalCardiovascular Research
Volume27
Issue number2
DOIs
StatePublished - Jan 1 1993

Fingerprint

Thyroxine
Adrenergic Receptors
Heart Ventricles
Messenger RNA
Left Ventricular Hypertrophy
Gases
Genes
Pressure
Aortic Valve Stenosis
DNA Probes
Thyroid Hormones
Northern Blotting
Hormones
RNA
Gene Expression
Injections

Keywords

  • Cardiac hypertrophy
  • Gαs mRNA
  • Thyroxine.
  • β1 adrenergic receptor mRNA
  • β1 adrenergic receptors

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

β1 adrenergic receptor and gαs mRNAs in rat heart as a function of mechanical load and thyroxine intoxication. / Moalic, Jean Marie; Bourgeois, Francine; Mansier, Pascale; Machida, Curtis; Carré, François; Chevalier, Brigitte; Pitarque, Philippe; Swynghedauw, Bernard.

In: Cardiovascular Research, Vol. 27, No. 2, 01.01.1993, p. 231-237.

Research output: Contribution to journalArticle

Moalic, JM, Bourgeois, F, Mansier, P, Machida, C, Carré, F, Chevalier, B, Pitarque, P & Swynghedauw, B 1993, 'β1 adrenergic receptor and gαs mRNAs in rat heart as a function of mechanical load and thyroxine intoxication', Cardiovascular Research, vol. 27, no. 2, pp. 231-237. https://doi.org/10.1093/cvr/27.2.231
Moalic, Jean Marie ; Bourgeois, Francine ; Mansier, Pascale ; Machida, Curtis ; Carré, François ; Chevalier, Brigitte ; Pitarque, Philippe ; Swynghedauw, Bernard. / β1 adrenergic receptor and gαs mRNAs in rat heart as a function of mechanical load and thyroxine intoxication. In: Cardiovascular Research. 1993 ; Vol. 27, No. 2. pp. 231-237.
@article{8a9fd7802c4c4c94ac9407a515a185be,
title = "β1 adrenergic receptor and gαs mRNAs in rat heart as a function of mechanical load and thyroxine intoxication",
abstract = "Objective: The aim of this study was to determine the expression of genes coding for the β1 adrenergic receptor and the α subunit of Gs in the adult rat normal and hypertrophied left ventricle, and in the left ventricle of the hypophysectomised rat after T4 intoxication. Methods: Total RNA was extracted from normal, control, or hypertrophied left ventricles 5 weeks after aortic stenosis, and from left ventricles of control or T4 injected hypophysectomised animals. The expression of β1 adrenergic receptor and Gαas mRNAs was quantitated by northern blot analysis and hybridisation with specific 32P-dCTP labelled DNA probes. Results: β1 Adrenergic receptor mRNA was decreased (by 33{\%}) in compensated left ventricular hypertrophy without modification of the relative level of Gαs mRNA. The relative level of β1 adrenergic receptor mRNA correlated negatively with the degree of left ventricular hypertrophy, suggesting that the expression of the β1 adrenergic receptor gene is not activated by pressure overload. In the left ventricle of the hypophysectomised rat, a rapid increase in β1 adrenergic receptor mRNA (by 180{\%} 3 h after hormone injection) was observed in response to T4, with no change in the relative content of Gαs mRNA. These results provide evidence that β1 adrenergic receptor mRNA and Gas mRNA accumulate to different levels of abundance in the adult left ventricle, as indicated by their ratios (0.053 and 0.043 in sham operated and hypertrophied left ventricles respectively). This suggests that distinct mechanisms are involved in the control of the accumulation of these two mRNAs in cardiac tissue. Conclusions: The reduction in β1 adrenergic receptor density in the hypertrophied rat left ventricle is associated with a parallel reduction in the level of β1 adrenergic receptor mRNA. The β1 adrenergic receptor gene may belong to a group of genes which are not activated by pressure overload, but are responsive to thyroid hormone.Cardiovascular Research 1993;27:231-237.",
keywords = "Cardiac hypertrophy, Gαs mRNA, Thyroxine., β1 adrenergic receptor mRNA, β1 adrenergic receptors",
author = "Moalic, {Jean Marie} and Francine Bourgeois and Pascale Mansier and Curtis Machida and Fran{\cc}ois Carr{\'e} and Brigitte Chevalier and Philippe Pitarque and Bernard Swynghedauw",
year = "1993",
month = "1",
day = "1",
doi = "10.1093/cvr/27.2.231",
language = "English (US)",
volume = "27",
pages = "231--237",
journal = "Cardiovascular Research",
issn = "0008-6363",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - β1 adrenergic receptor and gαs mRNAs in rat heart as a function of mechanical load and thyroxine intoxication

AU - Moalic, Jean Marie

AU - Bourgeois, Francine

AU - Mansier, Pascale

AU - Machida, Curtis

AU - Carré, François

AU - Chevalier, Brigitte

AU - Pitarque, Philippe

AU - Swynghedauw, Bernard

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Objective: The aim of this study was to determine the expression of genes coding for the β1 adrenergic receptor and the α subunit of Gs in the adult rat normal and hypertrophied left ventricle, and in the left ventricle of the hypophysectomised rat after T4 intoxication. Methods: Total RNA was extracted from normal, control, or hypertrophied left ventricles 5 weeks after aortic stenosis, and from left ventricles of control or T4 injected hypophysectomised animals. The expression of β1 adrenergic receptor and Gαas mRNAs was quantitated by northern blot analysis and hybridisation with specific 32P-dCTP labelled DNA probes. Results: β1 Adrenergic receptor mRNA was decreased (by 33%) in compensated left ventricular hypertrophy without modification of the relative level of Gαs mRNA. The relative level of β1 adrenergic receptor mRNA correlated negatively with the degree of left ventricular hypertrophy, suggesting that the expression of the β1 adrenergic receptor gene is not activated by pressure overload. In the left ventricle of the hypophysectomised rat, a rapid increase in β1 adrenergic receptor mRNA (by 180% 3 h after hormone injection) was observed in response to T4, with no change in the relative content of Gαs mRNA. These results provide evidence that β1 adrenergic receptor mRNA and Gas mRNA accumulate to different levels of abundance in the adult left ventricle, as indicated by their ratios (0.053 and 0.043 in sham operated and hypertrophied left ventricles respectively). This suggests that distinct mechanisms are involved in the control of the accumulation of these two mRNAs in cardiac tissue. Conclusions: The reduction in β1 adrenergic receptor density in the hypertrophied rat left ventricle is associated with a parallel reduction in the level of β1 adrenergic receptor mRNA. The β1 adrenergic receptor gene may belong to a group of genes which are not activated by pressure overload, but are responsive to thyroid hormone.Cardiovascular Research 1993;27:231-237.

AB - Objective: The aim of this study was to determine the expression of genes coding for the β1 adrenergic receptor and the α subunit of Gs in the adult rat normal and hypertrophied left ventricle, and in the left ventricle of the hypophysectomised rat after T4 intoxication. Methods: Total RNA was extracted from normal, control, or hypertrophied left ventricles 5 weeks after aortic stenosis, and from left ventricles of control or T4 injected hypophysectomised animals. The expression of β1 adrenergic receptor and Gαas mRNAs was quantitated by northern blot analysis and hybridisation with specific 32P-dCTP labelled DNA probes. Results: β1 Adrenergic receptor mRNA was decreased (by 33%) in compensated left ventricular hypertrophy without modification of the relative level of Gαs mRNA. The relative level of β1 adrenergic receptor mRNA correlated negatively with the degree of left ventricular hypertrophy, suggesting that the expression of the β1 adrenergic receptor gene is not activated by pressure overload. In the left ventricle of the hypophysectomised rat, a rapid increase in β1 adrenergic receptor mRNA (by 180% 3 h after hormone injection) was observed in response to T4, with no change in the relative content of Gαs mRNA. These results provide evidence that β1 adrenergic receptor mRNA and Gas mRNA accumulate to different levels of abundance in the adult left ventricle, as indicated by their ratios (0.053 and 0.043 in sham operated and hypertrophied left ventricles respectively). This suggests that distinct mechanisms are involved in the control of the accumulation of these two mRNAs in cardiac tissue. Conclusions: The reduction in β1 adrenergic receptor density in the hypertrophied rat left ventricle is associated with a parallel reduction in the level of β1 adrenergic receptor mRNA. The β1 adrenergic receptor gene may belong to a group of genes which are not activated by pressure overload, but are responsive to thyroid hormone.Cardiovascular Research 1993;27:231-237.

KW - Cardiac hypertrophy

KW - Gαs mRNA

KW - Thyroxine.

KW - β1 adrenergic receptor mRNA

KW - β1 adrenergic receptors

UR - http://www.scopus.com/inward/record.url?scp=85047676501&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047676501&partnerID=8YFLogxK

U2 - 10.1093/cvr/27.2.231

DO - 10.1093/cvr/27.2.231

M3 - Article

C2 - 8386062

AN - SCOPUS:85047676501

VL - 27

SP - 231

EP - 237

JO - Cardiovascular Research

JF - Cardiovascular Research

SN - 0008-6363

IS - 2

ER -