β2-Microglobulin from serum associates with several class I antigens expressed on the surface of mouse L-cells

Carmelo Bernabeu, Richard Maziarz, Cornelis Murre, Cox Terhorst

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Bovine β2-microglobulin (β2-m) present in fetal calf serum (FCS) is able to replace endogenous β2-m associated with several class I antigens from human and mouse cells maintained in culture [Bernabeu et al. (1984) Nature, Lond. 308, 642-645]. Here we show that human HLA-A2 and HLA-B7, as well as mouse H-2Ld and H-2Dd heavy chains expressed after gene transfer in mouse L-cells, associate to a large extent with bovine β2-m. We also demonstrate that bovine β2-m associated with the endogenous H-2Kk/Dk heavy chains generates an antibody response when L-cells are injected into syngeneic C3H mice.

Original languageEnglish (US)
Pages (from-to)955-960
Number of pages6
JournalMolecular Immunology
Volume22
Issue number8
DOIs
StatePublished - 1985
Externally publishedYes

Fingerprint

Histocompatibility Antigens Class I
Serum
HLA-B7 Antigen
HLA-A2 Antigen
Inbred C3H Mouse
Antibody Formation
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

β2-Microglobulin from serum associates with several class I antigens expressed on the surface of mouse L-cells. / Bernabeu, Carmelo; Maziarz, Richard; Murre, Cornelis; Terhorst, Cox.

In: Molecular Immunology, Vol. 22, No. 8, 1985, p. 955-960.

Research output: Contribution to journalArticle

@article{8c74c814259b4558bb554bd4ddfc82c9,
title = "β2-Microglobulin from serum associates with several class I antigens expressed on the surface of mouse L-cells",
abstract = "Bovine β2-microglobulin (β2-m) present in fetal calf serum (FCS) is able to replace endogenous β2-m associated with several class I antigens from human and mouse cells maintained in culture [Bernabeu et al. (1984) Nature, Lond. 308, 642-645]. Here we show that human HLA-A2 and HLA-B7, as well as mouse H-2Ld and H-2Dd heavy chains expressed after gene transfer in mouse L-cells, associate to a large extent with bovine β2-m. We also demonstrate that bovine β2-m associated with the endogenous H-2Kk/Dk heavy chains generates an antibody response when L-cells are injected into syngeneic C3H mice.",
author = "Carmelo Bernabeu and Richard Maziarz and Cornelis Murre and Cox Terhorst",
year = "1985",
doi = "10.1016/0161-5890(85)90082-3",
language = "English (US)",
volume = "22",
pages = "955--960",
journal = "Molecular Immunology",
issn = "0161-5890",
publisher = "Elsevier Limited",
number = "8",

}

TY - JOUR

T1 - β2-Microglobulin from serum associates with several class I antigens expressed on the surface of mouse L-cells

AU - Bernabeu, Carmelo

AU - Maziarz, Richard

AU - Murre, Cornelis

AU - Terhorst, Cox

PY - 1985

Y1 - 1985

N2 - Bovine β2-microglobulin (β2-m) present in fetal calf serum (FCS) is able to replace endogenous β2-m associated with several class I antigens from human and mouse cells maintained in culture [Bernabeu et al. (1984) Nature, Lond. 308, 642-645]. Here we show that human HLA-A2 and HLA-B7, as well as mouse H-2Ld and H-2Dd heavy chains expressed after gene transfer in mouse L-cells, associate to a large extent with bovine β2-m. We also demonstrate that bovine β2-m associated with the endogenous H-2Kk/Dk heavy chains generates an antibody response when L-cells are injected into syngeneic C3H mice.

AB - Bovine β2-microglobulin (β2-m) present in fetal calf serum (FCS) is able to replace endogenous β2-m associated with several class I antigens from human and mouse cells maintained in culture [Bernabeu et al. (1984) Nature, Lond. 308, 642-645]. Here we show that human HLA-A2 and HLA-B7, as well as mouse H-2Ld and H-2Dd heavy chains expressed after gene transfer in mouse L-cells, associate to a large extent with bovine β2-m. We also demonstrate that bovine β2-m associated with the endogenous H-2Kk/Dk heavy chains generates an antibody response when L-cells are injected into syngeneic C3H mice.

UR - http://www.scopus.com/inward/record.url?scp=0022003131&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022003131&partnerID=8YFLogxK

U2 - 10.1016/0161-5890(85)90082-3

DO - 10.1016/0161-5890(85)90082-3

M3 - Article

VL - 22

SP - 955

EP - 960

JO - Molecular Immunology

JF - Molecular Immunology

SN - 0161-5890

IS - 8

ER -