β-Endorphin and adrenocorticotropin in extrapituitary sites

Gastrointestinal tract

Eric Orwoll, John Kendall

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

β-Endorphin and ACTH immunoassays were employed to examine the concentrations, distributions, and character of those peptides in rat gastrointestinal tissues. Sections of the gastrointestinal tract were obtained from fasted and fed animals and were extracted in 5 N acetic acid containing proteolytic enzyme inhibitors. Aliquots immunoassayed for β-endorphin and ACTH revealed highest concentrations to be present in the small bowel, with stomach and colon containing little immunoreactivity. Tissues from fasted animals contained more immunoreactivity than did those from fed animals. Gel chromatography showed the presence of large molecular weight forms of β-endorphin and ACTH in gut extracts. Concanavalin A affinity chromatography revealed that approximately 5% of gut immunoreactivity contained carbohydrate. Therefore, β-endorphin and ACTH immunoreactivities are present in the gut. The demonstration of large molecular weight and glycosylated forms of immunoreactivity suggests the presence of biosynthetic precursors of β-endorphin and ACTH. The increase in immunoreactivity in response to fasting suggests that these peptides play a role in gut physiology.

Original languageEnglish (US)
Pages (from-to)438-442
Number of pages5
JournalEndocrinology
Volume107
Issue number2
StatePublished - 1980

Fingerprint

Endorphins
Adrenocorticotropic Hormone
Gastrointestinal Tract
Molecular Weight
Pro-Opiomelanocortin
Peptides
Enzyme Inhibitors
Concanavalin A
Affinity Chromatography
Immunoassay
Acetic Acid
Gel Chromatography
Fasting
Stomach
Colon
Peptide Hydrolases
Carbohydrates

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

β-Endorphin and adrenocorticotropin in extrapituitary sites : Gastrointestinal tract. / Orwoll, Eric; Kendall, John.

In: Endocrinology, Vol. 107, No. 2, 1980, p. 438-442.

Research output: Contribution to journalArticle

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