ß-glucan affects leukocyte navigation in a complex chemotactic gradient

Vassiliki Tsikitis, Jorge E. Albina, Jonathan S. Reichner

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background Polymorphonuclear leukocytes (PMNs) must traverse endogenous chemotactic gradients (interleukin 8 [IL-8]) before reaching target chemoattractants (fMLP [N-formylmethionine-leucine-phenylalanine], C5a) produced at a site of bacterial infection. Complement receptor 3 (CR3; CD11b/CD18) contains 2 distinct binding sites, one that mediates adhesion and a lectin-like domain (LLD) that binds polysaccharides of microbial origin. This laboratory previously reported an increase in the chemotactic capacity of PMNs toward fMLP upon ligation of the CR3 LLD with β-glucan, a CR3 agonist. Current studies sought to determine the effect of β-glucan on PMN navigation toward other chemoattractants alone and in a competing chemotactic environment. Methods Migration was assessed by serum agarose overlay with the use of chambered slides containing or not, β-glucan. Migration of human PMNs at 37°C for 2 hours was evaluated toward C5a or IL-8 alone and in competing gradients. Selected groups were treated with anti-CR3-blocking antibodies. The number of chemotactic cells was quantified by microscopy. Results β-glucan significantly enhanced chemotaxis toward C5a and suppressed that toward IL-8 in a CR3-dependent fashion. In the competing chemotactic gradient assays (C5a vs IL-8), β-glucan further enhanced migration toward C5a while not affecting that toward IL-8. Conclusions β-glucan selectively upregulates PMN chemotaxis toward C5a while suppressing chemotaxis toward IL-8.

Original languageEnglish (US)
Pages (from-to)384-389
Number of pages6
JournalSurgery
Volume136
Issue number2
DOIs
StatePublished - Aug 2004
Externally publishedYes

Fingerprint

Glucans
Interleukin-8
Leukocytes
Chemotaxis
Chemotactic Factors
Lectins
N-Formylmethionine
Macrophage-1 Antigen
Blocking Antibodies
Phenylalanine
Bacterial Infections
Leucine
Sepharose
Polysaccharides
Ligation
Microscopy
Neutrophils
Up-Regulation
Cell Count
Binding Sites

ASJC Scopus subject areas

  • Surgery

Cite this

ß-glucan affects leukocyte navigation in a complex chemotactic gradient. / Tsikitis, Vassiliki; Albina, Jorge E.; Reichner, Jonathan S.

In: Surgery, Vol. 136, No. 2, 08.2004, p. 384-389.

Research output: Contribution to journalArticle

Tsikitis, Vassiliki ; Albina, Jorge E. ; Reichner, Jonathan S. / ß-glucan affects leukocyte navigation in a complex chemotactic gradient. In: Surgery. 2004 ; Vol. 136, No. 2. pp. 384-389.
@article{8b3d19fe2dc54013a1ffbfaa3122d4dc,
title = "{\ss}-glucan affects leukocyte navigation in a complex chemotactic gradient",
abstract = "Background Polymorphonuclear leukocytes (PMNs) must traverse endogenous chemotactic gradients (interleukin 8 [IL-8]) before reaching target chemoattractants (fMLP [N-formylmethionine-leucine-phenylalanine], C5a) produced at a site of bacterial infection. Complement receptor 3 (CR3; CD11b/CD18) contains 2 distinct binding sites, one that mediates adhesion and a lectin-like domain (LLD) that binds polysaccharides of microbial origin. This laboratory previously reported an increase in the chemotactic capacity of PMNs toward fMLP upon ligation of the CR3 LLD with β-glucan, a CR3 agonist. Current studies sought to determine the effect of β-glucan on PMN navigation toward other chemoattractants alone and in a competing chemotactic environment. Methods Migration was assessed by serum agarose overlay with the use of chambered slides containing or not, β-glucan. Migration of human PMNs at 37°C for 2 hours was evaluated toward C5a or IL-8 alone and in competing gradients. Selected groups were treated with anti-CR3-blocking antibodies. The number of chemotactic cells was quantified by microscopy. Results β-glucan significantly enhanced chemotaxis toward C5a and suppressed that toward IL-8 in a CR3-dependent fashion. In the competing chemotactic gradient assays (C5a vs IL-8), β-glucan further enhanced migration toward C5a while not affecting that toward IL-8. Conclusions β-glucan selectively upregulates PMN chemotaxis toward C5a while suppressing chemotaxis toward IL-8.",
author = "Vassiliki Tsikitis and Albina, {Jorge E.} and Reichner, {Jonathan S.}",
year = "2004",
month = "8",
doi = "10.1016/j.surg.2004.05.014",
language = "English (US)",
volume = "136",
pages = "384--389",
journal = "Surgery (United States)",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "2",

}

TY - JOUR

T1 - ß-glucan affects leukocyte navigation in a complex chemotactic gradient

AU - Tsikitis, Vassiliki

AU - Albina, Jorge E.

AU - Reichner, Jonathan S.

PY - 2004/8

Y1 - 2004/8

N2 - Background Polymorphonuclear leukocytes (PMNs) must traverse endogenous chemotactic gradients (interleukin 8 [IL-8]) before reaching target chemoattractants (fMLP [N-formylmethionine-leucine-phenylalanine], C5a) produced at a site of bacterial infection. Complement receptor 3 (CR3; CD11b/CD18) contains 2 distinct binding sites, one that mediates adhesion and a lectin-like domain (LLD) that binds polysaccharides of microbial origin. This laboratory previously reported an increase in the chemotactic capacity of PMNs toward fMLP upon ligation of the CR3 LLD with β-glucan, a CR3 agonist. Current studies sought to determine the effect of β-glucan on PMN navigation toward other chemoattractants alone and in a competing chemotactic environment. Methods Migration was assessed by serum agarose overlay with the use of chambered slides containing or not, β-glucan. Migration of human PMNs at 37°C for 2 hours was evaluated toward C5a or IL-8 alone and in competing gradients. Selected groups were treated with anti-CR3-blocking antibodies. The number of chemotactic cells was quantified by microscopy. Results β-glucan significantly enhanced chemotaxis toward C5a and suppressed that toward IL-8 in a CR3-dependent fashion. In the competing chemotactic gradient assays (C5a vs IL-8), β-glucan further enhanced migration toward C5a while not affecting that toward IL-8. Conclusions β-glucan selectively upregulates PMN chemotaxis toward C5a while suppressing chemotaxis toward IL-8.

AB - Background Polymorphonuclear leukocytes (PMNs) must traverse endogenous chemotactic gradients (interleukin 8 [IL-8]) before reaching target chemoattractants (fMLP [N-formylmethionine-leucine-phenylalanine], C5a) produced at a site of bacterial infection. Complement receptor 3 (CR3; CD11b/CD18) contains 2 distinct binding sites, one that mediates adhesion and a lectin-like domain (LLD) that binds polysaccharides of microbial origin. This laboratory previously reported an increase in the chemotactic capacity of PMNs toward fMLP upon ligation of the CR3 LLD with β-glucan, a CR3 agonist. Current studies sought to determine the effect of β-glucan on PMN navigation toward other chemoattractants alone and in a competing chemotactic environment. Methods Migration was assessed by serum agarose overlay with the use of chambered slides containing or not, β-glucan. Migration of human PMNs at 37°C for 2 hours was evaluated toward C5a or IL-8 alone and in competing gradients. Selected groups were treated with anti-CR3-blocking antibodies. The number of chemotactic cells was quantified by microscopy. Results β-glucan significantly enhanced chemotaxis toward C5a and suppressed that toward IL-8 in a CR3-dependent fashion. In the competing chemotactic gradient assays (C5a vs IL-8), β-glucan further enhanced migration toward C5a while not affecting that toward IL-8. Conclusions β-glucan selectively upregulates PMN chemotaxis toward C5a while suppressing chemotaxis toward IL-8.

UR - http://www.scopus.com/inward/record.url?scp=3843080856&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3843080856&partnerID=8YFLogxK

U2 - 10.1016/j.surg.2004.05.014

DO - 10.1016/j.surg.2004.05.014

M3 - Article

C2 - 15300205

AN - SCOPUS:3843080856

VL - 136

SP - 384

EP - 389

JO - Surgery (United States)

JF - Surgery (United States)

SN - 0039-6060

IS - 2

ER -