Zika Virus infection of rhesus macaques leads to viral persistence in multiple tissues

Alec Hirsch, Jessica L. Smith, Nicole N. Haese, Rebecca M. Broeckel, Christopher J. Parkins, Craig Kreklywich, Victor De Filippis, Michael Denton, Patricia P. Smith, William Messer, Lois Colgin, Rebecca Ducore, Peta Grigsby, Jon Hennebold, Tonya Swanson, Alfred W. Legasse, Michael Axthelm, Rhonda MacAllister, Clayton A. Wiley, Jay NelsonDaniel Streblow

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Zika virus (ZIKV), an emerging flavivirus, has recently spread explosively through the Western hemisphere. In addition to symptoms including fever, rash, arthralgia, and conjunctivitis, ZIKV infection of pregnant women can cause microcephaly and other developmental abnormalities in the fetus. We report herein the results of ZIKV infection of adult rhesus macaques. Following subcutaneous infection, animals developed transient plasma viremia and viruria from 1–7 days post infection (dpi) that was accompanied by the development of a rash, fever and conjunctivitis. Animals produced a robust adaptive immune response to ZIKV, although systemic cytokine response was minimal. At 7 dpi, virus was detected in peripheral nervous tissue, multiple lymphoid tissues, joints, and the uterus of the necropsied animals. Notably, viral RNA persisted in neuronal, lymphoid and joint/muscle tissues and the male and female reproductive tissues through 28 to 35 dpi. The tropism and persistence of ZIKV in the peripheral nerves and reproductive tract may provide a mechanism of subsequent neuropathogenesis and sexual transmission.

Original languageEnglish (US)
Article numbere1006219
JournalPLoS Pathogens
Volume13
Issue number3
DOIs
StatePublished - Mar 1 2017

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Macaca mulatta
Conjunctivitis
Exanthema
Fever
Joints
Infection
Flavivirus
Nerve Tissue
Microcephaly
Tropism
Viremia
Viral RNA
Arthralgia
Adaptive Immunity
Lymphoid Tissue
Virus Diseases
Peripheral Nerves
Uterus
Pregnant Women
Fetus

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Zika Virus infection of rhesus macaques leads to viral persistence in multiple tissues. / Hirsch, Alec; Smith, Jessica L.; Haese, Nicole N.; Broeckel, Rebecca M.; Parkins, Christopher J.; Kreklywich, Craig; De Filippis, Victor; Denton, Michael; Smith, Patricia P.; Messer, William; Colgin, Lois; Ducore, Rebecca; Grigsby, Peta; Hennebold, Jon; Swanson, Tonya; Legasse, Alfred W.; Axthelm, Michael; MacAllister, Rhonda; Wiley, Clayton A.; Nelson, Jay; Streblow, Daniel.

In: PLoS Pathogens, Vol. 13, No. 3, e1006219, 01.03.2017.

Research output: Contribution to journalArticle

Hirsch, A, Smith, JL, Haese, NN, Broeckel, RM, Parkins, CJ, Kreklywich, C, De Filippis, V, Denton, M, Smith, PP, Messer, W, Colgin, L, Ducore, R, Grigsby, P, Hennebold, J, Swanson, T, Legasse, AW, Axthelm, M, MacAllister, R, Wiley, CA, Nelson, J & Streblow, D 2017, 'Zika Virus infection of rhesus macaques leads to viral persistence in multiple tissues', PLoS Pathogens, vol. 13, no. 3, e1006219. https://doi.org/10.1371/journal.ppat.1006219
Hirsch, Alec ; Smith, Jessica L. ; Haese, Nicole N. ; Broeckel, Rebecca M. ; Parkins, Christopher J. ; Kreklywich, Craig ; De Filippis, Victor ; Denton, Michael ; Smith, Patricia P. ; Messer, William ; Colgin, Lois ; Ducore, Rebecca ; Grigsby, Peta ; Hennebold, Jon ; Swanson, Tonya ; Legasse, Alfred W. ; Axthelm, Michael ; MacAllister, Rhonda ; Wiley, Clayton A. ; Nelson, Jay ; Streblow, Daniel. / Zika Virus infection of rhesus macaques leads to viral persistence in multiple tissues. In: PLoS Pathogens. 2017 ; Vol. 13, No. 3.
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