Zebrafish dorsal root ganglia neural precursor cells adopt a glial fate in the absence of neurogenin1

Hillary F. McGraw, Alex Nechiporuk, David W. Raible

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The proneural transcription factor neurogenin 1 (neurog1) has been shown to be a key regulator of dorsal root ganglion (DRG) neuron development. Here we use a novel transgenic zebrafish line to demonstrate that the neural crest population that gives rise to DRG neurons becomes fate restricted to a neuronal/glial precursor before the onset of neurog1 function. We generated a stable transgenic zebrafish line that carries a modified bacterial artificial chromosome that expresses green fluorescent protein (GFP) under the control of the neurog1 promoter [Tg(neurog1:EGFP)]. In contrast to previously described neurog1 transgenic lines, Tg(neurog1:EGFP) expresses GFP in DRG neuronal precursors cells as they migrate ventrally and after their initial differentiation as neurons. Using this line, we are able to track the fate of DRG neuronal precursor cells during their specification. When Neurog1 function is blocked, either by neurog1 morpholino antisense oligonucleotide injection or in neurog1 mutants, GFP expression initiates in neural crest cells, although they fail to form DRG neurons. Rather, these cells take on a glial-like morphology, retain proliferative capacity, and express glial markers and become associated with the ventral motor root. These results suggest that, within the zebrafish neural crest, there is a fate-restricted lineage that is limited to form either sensory neurons or glia in the developing DRG. Neurog1 acts as the key factor in this lineage to direct the formation of sensory neurons.

Original languageEnglish (US)
Pages (from-to)12558-12569
Number of pages12
JournalJournal of Neuroscience
Volume28
Issue number47
DOIs
StatePublished - Nov 19 2008

Fingerprint

Spinal Ganglia
Zebrafish
Neuroglia
Neural Crest
Green Fluorescent Proteins
Neurons
Sensory Receptor Cells
Bacterial Artificial Chromosomes
Morpholinos
Spinal Nerve Roots
Antisense Oligonucleotides
Transcription Factors
Injections
Population

Keywords

  • Dorsal root ganglia
  • Fate restriction
  • Glia
  • Neural crest
  • Neurogenin 1
  • Sensory neuron

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Zebrafish dorsal root ganglia neural precursor cells adopt a glial fate in the absence of neurogenin1. / McGraw, Hillary F.; Nechiporuk, Alex; Raible, David W.

In: Journal of Neuroscience, Vol. 28, No. 47, 19.11.2008, p. 12558-12569.

Research output: Contribution to journalArticle

@article{21b586a731d244fc82dade9207b333d3,
title = "Zebrafish dorsal root ganglia neural precursor cells adopt a glial fate in the absence of neurogenin1",
abstract = "The proneural transcription factor neurogenin 1 (neurog1) has been shown to be a key regulator of dorsal root ganglion (DRG) neuron development. Here we use a novel transgenic zebrafish line to demonstrate that the neural crest population that gives rise to DRG neurons becomes fate restricted to a neuronal/glial precursor before the onset of neurog1 function. We generated a stable transgenic zebrafish line that carries a modified bacterial artificial chromosome that expresses green fluorescent protein (GFP) under the control of the neurog1 promoter [Tg(neurog1:EGFP)]. In contrast to previously described neurog1 transgenic lines, Tg(neurog1:EGFP) expresses GFP in DRG neuronal precursors cells as they migrate ventrally and after their initial differentiation as neurons. Using this line, we are able to track the fate of DRG neuronal precursor cells during their specification. When Neurog1 function is blocked, either by neurog1 morpholino antisense oligonucleotide injection or in neurog1 mutants, GFP expression initiates in neural crest cells, although they fail to form DRG neurons. Rather, these cells take on a glial-like morphology, retain proliferative capacity, and express glial markers and become associated with the ventral motor root. These results suggest that, within the zebrafish neural crest, there is a fate-restricted lineage that is limited to form either sensory neurons or glia in the developing DRG. Neurog1 acts as the key factor in this lineage to direct the formation of sensory neurons.",
keywords = "Dorsal root ganglia, Fate restriction, Glia, Neural crest, Neurogenin 1, Sensory neuron",
author = "McGraw, {Hillary F.} and Alex Nechiporuk and Raible, {David W.}",
year = "2008",
month = "11",
day = "19",
doi = "10.1523/JNEUROSCI.2079-08.2008",
language = "English (US)",
volume = "28",
pages = "12558--12569",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "47",

}

TY - JOUR

T1 - Zebrafish dorsal root ganglia neural precursor cells adopt a glial fate in the absence of neurogenin1

AU - McGraw, Hillary F.

AU - Nechiporuk, Alex

AU - Raible, David W.

PY - 2008/11/19

Y1 - 2008/11/19

N2 - The proneural transcription factor neurogenin 1 (neurog1) has been shown to be a key regulator of dorsal root ganglion (DRG) neuron development. Here we use a novel transgenic zebrafish line to demonstrate that the neural crest population that gives rise to DRG neurons becomes fate restricted to a neuronal/glial precursor before the onset of neurog1 function. We generated a stable transgenic zebrafish line that carries a modified bacterial artificial chromosome that expresses green fluorescent protein (GFP) under the control of the neurog1 promoter [Tg(neurog1:EGFP)]. In contrast to previously described neurog1 transgenic lines, Tg(neurog1:EGFP) expresses GFP in DRG neuronal precursors cells as they migrate ventrally and after their initial differentiation as neurons. Using this line, we are able to track the fate of DRG neuronal precursor cells during their specification. When Neurog1 function is blocked, either by neurog1 morpholino antisense oligonucleotide injection or in neurog1 mutants, GFP expression initiates in neural crest cells, although they fail to form DRG neurons. Rather, these cells take on a glial-like morphology, retain proliferative capacity, and express glial markers and become associated with the ventral motor root. These results suggest that, within the zebrafish neural crest, there is a fate-restricted lineage that is limited to form either sensory neurons or glia in the developing DRG. Neurog1 acts as the key factor in this lineage to direct the formation of sensory neurons.

AB - The proneural transcription factor neurogenin 1 (neurog1) has been shown to be a key regulator of dorsal root ganglion (DRG) neuron development. Here we use a novel transgenic zebrafish line to demonstrate that the neural crest population that gives rise to DRG neurons becomes fate restricted to a neuronal/glial precursor before the onset of neurog1 function. We generated a stable transgenic zebrafish line that carries a modified bacterial artificial chromosome that expresses green fluorescent protein (GFP) under the control of the neurog1 promoter [Tg(neurog1:EGFP)]. In contrast to previously described neurog1 transgenic lines, Tg(neurog1:EGFP) expresses GFP in DRG neuronal precursors cells as they migrate ventrally and after their initial differentiation as neurons. Using this line, we are able to track the fate of DRG neuronal precursor cells during their specification. When Neurog1 function is blocked, either by neurog1 morpholino antisense oligonucleotide injection or in neurog1 mutants, GFP expression initiates in neural crest cells, although they fail to form DRG neurons. Rather, these cells take on a glial-like morphology, retain proliferative capacity, and express glial markers and become associated with the ventral motor root. These results suggest that, within the zebrafish neural crest, there is a fate-restricted lineage that is limited to form either sensory neurons or glia in the developing DRG. Neurog1 acts as the key factor in this lineage to direct the formation of sensory neurons.

KW - Dorsal root ganglia

KW - Fate restriction

KW - Glia

KW - Neural crest

KW - Neurogenin 1

KW - Sensory neuron

UR - http://www.scopus.com/inward/record.url?scp=58149377163&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149377163&partnerID=8YFLogxK

U2 - 10.1523/JNEUROSCI.2079-08.2008

DO - 10.1523/JNEUROSCI.2079-08.2008

M3 - Article

VL - 28

SP - 12558

EP - 12569

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 47

ER -