Xenopus laevis contains two nonallelic preproinsulin genes. cDNA cloning and evolutionary perspective

A. R. Shuldiner, S. Phillips, Charles Roberts, D. LeRoith, J. Roth

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We undertook the cloning of preproinsulin cDNAs from the South African clawed toad, Xenopus laevis, in order to study the role of insulin during embryogenesis in this species. We found that X. laevis contains two different preproinsulin cDNAs, both of which code for peptides containing 106 amino acids of typical structure but which differ by eight amino acids: one in the signal peptide, two in the B-chain, four in the C-peptide, and one in the A-chain. Southern blot analysis indicates that the two preproinsulin cDNAs identified correspond to two different nonallelic genes which we believe arose through a recent gene duplication within the amphibian radiation possibly during the development of tetraploidy in this species. Both genes are expressed, since we have recently identified the two corresponding insulins in pancreatic extracts of adult toads (Shuldiner, A.R., Bennett, C., Robinson, E.A., and Roth, J. (1989) Endocrinology, in press). These cDNAs represent the first amphibian preproinsulin sequences to be elucidated.

Original languageEnglish (US)
Pages (from-to)9428-9432
Number of pages5
JournalJournal of Biological Chemistry
Volume264
Issue number16
StatePublished - 1989
Externally publishedYes

Fingerprint

Cloning
Xenopus laevis
Organism Cloning
Complementary DNA
Genes
Amphibians
Anura
Endocrinology
Pancreatic Extracts
Insulins
Amino Acids
Gene Duplication
Tetraploidy
C-Peptide
Protein Sorting Signals
Southern Blotting
Embryonic Development
Insulin
Radiation
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Xenopus laevis contains two nonallelic preproinsulin genes. cDNA cloning and evolutionary perspective. / Shuldiner, A. R.; Phillips, S.; Roberts, Charles; LeRoith, D.; Roth, J.

In: Journal of Biological Chemistry, Vol. 264, No. 16, 1989, p. 9428-9432.

Research output: Contribution to journalArticle

Shuldiner, A. R. ; Phillips, S. ; Roberts, Charles ; LeRoith, D. ; Roth, J. / Xenopus laevis contains two nonallelic preproinsulin genes. cDNA cloning and evolutionary perspective. In: Journal of Biological Chemistry. 1989 ; Vol. 264, No. 16. pp. 9428-9432.
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