XeCI laser-induced fluorescence detection of peroxidized lipoproteins in lipid-rich atherosclerotic lesions

A. A. Oraevsky, P. D. Henry, S. L. Jacques, F. K. Titte

Research output: Contribution to journalConference article

Abstract

Laser-induced fluorescence spectroscopy of arterial surfaces provides information about the composition of atherosclerotic plaques. The aim of the study was to determine whether accumulation of peroxidized lipoproteins in arterial walls, a process postulated to play a role in initiating atherosclerotic changes, can be demonstrated by fluorescence spectroscopy. XeC1 excimer laser (= 308 nm) induced fluorescence of human aortas containing early lipid-rich, non-collagenous lesions exhibited marked red shifts and broadening of the fluorescence spectra compared with spectra from non-atherosclerotic aortas. Similar profiles were observed in spectra obtained from oxidatively modified LDL, but not native LDL. In hypercholesterolemic rabbits with early foam cell lesions, spectral shifts resembled those of oxidized f3-VLDL, the major lipoprotein accumulating in arteries of rabbits fed cholesterol. XeCl laser-fluorescence spectroscopy of arterial surfaces may be useful for the identification of arterial plaques indicative of atherosclerosis in its early and probably reversible stages.

Original languageEnglish (US)
Pages (from-to)31-41
Number of pages11
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume1878
DOIs
StatePublished - Jun 23 1993
EventDiagnostic and Therapeutic Cardiovascular Interventions III 1993 - Los Angeles, United States
Duration: Jan 17 1993Jan 22 1993

Fingerprint

lipoproteins
Fluorescence Spectroscopy
Laser-induced Fluorescence
Lipoproteins
Fluorescence spectroscopy
Lipids
laser induced fluorescence
lesions
lipids
Aorta
Fluorescence
Rabbit
aorta
fluorescence
Lasers
rabbits
Laser Spectroscopy
Atherosclerosis
Excimer Laser
Laser spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

XeCI laser-induced fluorescence detection of peroxidized lipoproteins in lipid-rich atherosclerotic lesions. / Oraevsky, A. A.; Henry, P. D.; Jacques, S. L.; Titte, F. K.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 1878, 23.06.1993, p. 31-41.

Research output: Contribution to journalConference article

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AU - Titte, F. K.

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N2 - Laser-induced fluorescence spectroscopy of arterial surfaces provides information about the composition of atherosclerotic plaques. The aim of the study was to determine whether accumulation of peroxidized lipoproteins in arterial walls, a process postulated to play a role in initiating atherosclerotic changes, can be demonstrated by fluorescence spectroscopy. XeC1 excimer laser (= 308 nm) induced fluorescence of human aortas containing early lipid-rich, non-collagenous lesions exhibited marked red shifts and broadening of the fluorescence spectra compared with spectra from non-atherosclerotic aortas. Similar profiles were observed in spectra obtained from oxidatively modified LDL, but not native LDL. In hypercholesterolemic rabbits with early foam cell lesions, spectral shifts resembled those of oxidized f3-VLDL, the major lipoprotein accumulating in arteries of rabbits fed cholesterol. XeCl laser-fluorescence spectroscopy of arterial surfaces may be useful for the identification of arterial plaques indicative of atherosclerosis in its early and probably reversible stages.

AB - Laser-induced fluorescence spectroscopy of arterial surfaces provides information about the composition of atherosclerotic plaques. The aim of the study was to determine whether accumulation of peroxidized lipoproteins in arterial walls, a process postulated to play a role in initiating atherosclerotic changes, can be demonstrated by fluorescence spectroscopy. XeC1 excimer laser (= 308 nm) induced fluorescence of human aortas containing early lipid-rich, non-collagenous lesions exhibited marked red shifts and broadening of the fluorescence spectra compared with spectra from non-atherosclerotic aortas. Similar profiles were observed in spectra obtained from oxidatively modified LDL, but not native LDL. In hypercholesterolemic rabbits with early foam cell lesions, spectral shifts resembled those of oxidized f3-VLDL, the major lipoprotein accumulating in arteries of rabbits fed cholesterol. XeCl laser-fluorescence spectroscopy of arterial surfaces may be useful for the identification of arterial plaques indicative of atherosclerosis in its early and probably reversible stages.

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