Xanthohumol inhibits the neuroendocrine transcription factor achaete-scute complex-like 1, suppresses proliferation, and induces phosphorylated ERK1/2 in medullary thyroid cancer

Mackenzie R. Cook, Jie Luo, Mary Ndiaye, Herbert Chen, Muthusamy Kunnimalaiyaan

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Background: Achaete-scute complex-like 1 (ASCL1) is a transcription factor important in the malignant development of medullary thyroid cancer (MTC). Activation of Raf-1 signaling is associated with ASCL1 suppression and growth inhibition. Xanthohumol, a natural compound, has recently been shown to have anticancer properties. We thus hypothesized that xanthohumol would suppress growth by activating Raf-1 signaling, thus altering the malignant phenotype of MTC. Methods: Human MTC cells were treated with xanthohumol (0-30 μmol/L) for up to 6 days. Proliferation was measured by a methylthiazolyldiphenyl-tetrazolium bromide (MTT) colorimetric assay. Western blot analysis was performed for ASCL1 and markers of Raf-1 pathway activation. Results: Treatment of MTC cells with xanthohumol resulted in a dose dependent inhibition of growth. Additionally, induction of phosphorylated ERK1/2 and a reduction of ASCL1 protein was noted. Conclusions: Xanthohumol is a potent Raf-1 activator in MTC cells. This compound suppresses MTC growth, alters the malignant phenotype, and warrants further preclinical study.

Original languageEnglish (US)
Pages (from-to)315-318
Number of pages4
JournalAmerican journal of surgery
Volume199
Issue number3
DOIs
StatePublished - Mar 2010
Externally publishedYes

Keywords

  • Achaete-scute complex-like 1
  • Medullary thyroid cancer
  • Neuroendocrine tumor
  • Phosphorylated ERK1/2
  • Xanthohumol

ASJC Scopus subject areas

  • Surgery

Fingerprint Dive into the research topics of 'Xanthohumol inhibits the neuroendocrine transcription factor achaete-scute complex-like 1, suppresses proliferation, and induces phosphorylated ERK1/2 in medullary thyroid cancer'. Together they form a unique fingerprint.

Cite this