X-ray laser microscopy of rat sperm nuclei

L. B. Da Silva, J. E. Trebes, R. Balhorn, S. Mrowka, E. Anderson, D. T. Attwood, T. W. Barbee, J. Brase, M. Corzett, Joe Gray, J. A. Koch, C. Lee, D. Kern, R. A. London, B. J. MacGowan, D. L. Matthews, G. Stone

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

The development of high brightness and short pulse width (<200 picoseconds) x-ray lasers now offers biologists the possibility of high-resolution imaging of specimens in an aqueous environment without the blurring effects associated with natural motions and chemical erosion. As a step toward developing the capabilities of this type of x-ray microscopy, a tantalum x-ray laser at 44.83 angstrom wavelength was used together with an x-ray zone plate lens to image both unlabeled and selectively gold-labeled dried rat sperm nuclei. The observed images show ∼500 angstrom features, illustrate the importance of x-ray microscopy in determining chemical composition, and provide information about the uniformity of sperm chromatin organization and the extent of sperm chromatin hydration.

Original languageEnglish (US)
Pages (from-to)269-271
Number of pages3
JournalScience
Volume258
Issue number5080
StatePublished - Oct 9 1992
Externally publishedYes

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Confocal Microscopy
Spermatozoa
X-Rays
Chromatin
Microscopy
Lasers
Tantalum
Gold
Lenses

ASJC Scopus subject areas

  • General

Cite this

Da Silva, L. B., Trebes, J. E., Balhorn, R., Mrowka, S., Anderson, E., Attwood, D. T., ... Stone, G. (1992). X-ray laser microscopy of rat sperm nuclei. Science, 258(5080), 269-271.

X-ray laser microscopy of rat sperm nuclei. / Da Silva, L. B.; Trebes, J. E.; Balhorn, R.; Mrowka, S.; Anderson, E.; Attwood, D. T.; Barbee, T. W.; Brase, J.; Corzett, M.; Gray, Joe; Koch, J. A.; Lee, C.; Kern, D.; London, R. A.; MacGowan, B. J.; Matthews, D. L.; Stone, G.

In: Science, Vol. 258, No. 5080, 09.10.1992, p. 269-271.

Research output: Contribution to journalArticle

Da Silva, LB, Trebes, JE, Balhorn, R, Mrowka, S, Anderson, E, Attwood, DT, Barbee, TW, Brase, J, Corzett, M, Gray, J, Koch, JA, Lee, C, Kern, D, London, RA, MacGowan, BJ, Matthews, DL & Stone, G 1992, 'X-ray laser microscopy of rat sperm nuclei', Science, vol. 258, no. 5080, pp. 269-271.
Da Silva LB, Trebes JE, Balhorn R, Mrowka S, Anderson E, Attwood DT et al. X-ray laser microscopy of rat sperm nuclei. Science. 1992 Oct 9;258(5080):269-271.
Da Silva, L. B. ; Trebes, J. E. ; Balhorn, R. ; Mrowka, S. ; Anderson, E. ; Attwood, D. T. ; Barbee, T. W. ; Brase, J. ; Corzett, M. ; Gray, Joe ; Koch, J. A. ; Lee, C. ; Kern, D. ; London, R. A. ; MacGowan, B. J. ; Matthews, D. L. ; Stone, G. / X-ray laser microscopy of rat sperm nuclei. In: Science. 1992 ; Vol. 258, No. 5080. pp. 269-271.
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