X-ray laser microscopy of rat sperm nuclei

L. B. Da Silva; J. E. Trebes; R. Balhorn; S. Mrowka; E. Anderson; D. T. Attwood; T. W. Barbee; J. Brase; M. Corzett; J. Gray; J. A. Koch; C. Lee; D. Kern; R. A. London; B. J. MacGowan; D. L. Matthews; G. Stone

doi:10.1126/science.1411525

X-ray laser microscopy of rat sperm nuclei

L. B. Da Silva, J. E. Trebes, R. Balhorn, S. Mrowka, E. Anderson, D. T. Attwood, T. W. Barbee, J. Brase, M. Corzett, J. Gray, J. A. Koch, C. Lee, D. Kern, R. A. London, B. J. MacGowan, D. L. Matthews, G. Stone

Research output: Contribution to journal › Article › peer-review

137 Scopus citations

Abstract

The development of high brightness and short pulse width (<200 picoseconds) x-ray lasers now offers biologists the possibility of high-resolution imaging of specimens in an aqueous environment without the blurring effects associated with natural motions and chemical erosion. As a step toward developing the capabilities of this type of x-ray microscopy, a tantalum x-ray laser at 44.83 angstrom wavelength was used together with an x-ray zone plate lens to image both unlabeled and selectively gold-labeled dried rat sperm nuclei. The observed images show ∼500 angstrom features, illustrate the importance of x-ray microscopy in determining chemical composition, and provide information about the uniformity of sperm chromatin organization and the extent of sperm chromatin hydration.

Original language	English (US)
Pages (from-to)	269-271
Number of pages	3
Journal	Science
Volume	258
Issue number	5080
DOIs	https://doi.org/10.1126/science.1411525
State	Published - 1992
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1126/science.1411525

Cite this

@article{232b19aae6c745fca4c4d68ae136c67f,

title = "X-ray laser microscopy of rat sperm nuclei",

abstract = "The development of high brightness and short pulse width (<200 picoseconds) x-ray lasers now offers biologists the possibility of high-resolution imaging of specimens in an aqueous environment without the blurring effects associated with natural motions and chemical erosion. As a step toward developing the capabilities of this type of x-ray microscopy, a tantalum x-ray laser at 44.83 angstrom wavelength was used together with an x-ray zone plate lens to image both unlabeled and selectively gold-labeled dried rat sperm nuclei. The observed images show ∼500 angstrom features, illustrate the importance of x-ray microscopy in determining chemical composition, and provide information about the uniformity of sperm chromatin organization and the extent of sperm chromatin hydration.",

author = "{Da Silva}, {L. B.} and Trebes, {J. E.} and R. Balhorn and S. Mrowka and E. Anderson and Attwood, {D. T.} and Barbee, {T. W.} and J. Brase and M. Corzett and J. Gray and Koch, {J. A.} and C. Lee and D. Kern and London, {R. A.} and MacGowan, {B. J.} and Matthews, {D. L.} and G. Stone",

year = "1992",

doi = "10.1126/science.1411525",

language = "English (US)",

volume = "258",

pages = "269--271",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5080",

}

TY - JOUR

T1 - X-ray laser microscopy of rat sperm nuclei

AU - Da Silva, L. B.

AU - Trebes, J. E.

AU - Balhorn, R.

AU - Mrowka, S.

AU - Anderson, E.

AU - Attwood, D. T.

AU - Barbee, T. W.

AU - Brase, J.

AU - Corzett, M.

AU - Gray, J.

AU - Koch, J. A.

AU - Lee, C.

AU - Kern, D.

AU - London, R. A.

AU - MacGowan, B. J.

AU - Matthews, D. L.

AU - Stone, G.

PY - 1992

Y1 - 1992

N2 - The development of high brightness and short pulse width (<200 picoseconds) x-ray lasers now offers biologists the possibility of high-resolution imaging of specimens in an aqueous environment without the blurring effects associated with natural motions and chemical erosion. As a step toward developing the capabilities of this type of x-ray microscopy, a tantalum x-ray laser at 44.83 angstrom wavelength was used together with an x-ray zone plate lens to image both unlabeled and selectively gold-labeled dried rat sperm nuclei. The observed images show ∼500 angstrom features, illustrate the importance of x-ray microscopy in determining chemical composition, and provide information about the uniformity of sperm chromatin organization and the extent of sperm chromatin hydration.

AB - The development of high brightness and short pulse width (<200 picoseconds) x-ray lasers now offers biologists the possibility of high-resolution imaging of specimens in an aqueous environment without the blurring effects associated with natural motions and chemical erosion. As a step toward developing the capabilities of this type of x-ray microscopy, a tantalum x-ray laser at 44.83 angstrom wavelength was used together with an x-ray zone plate lens to image both unlabeled and selectively gold-labeled dried rat sperm nuclei. The observed images show ∼500 angstrom features, illustrate the importance of x-ray microscopy in determining chemical composition, and provide information about the uniformity of sperm chromatin organization and the extent of sperm chromatin hydration.

UR - http://www.scopus.com/inward/record.url?scp=0026495807&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026495807&partnerID=8YFLogxK

U2 - 10.1126/science.1411525

DO - 10.1126/science.1411525

M3 - Article

C2 - 1411525

AN - SCOPUS:0026495807

SN - 0036-8075

VL - 258

SP - 269

EP - 271

JO - Science

JF - Science

IS - 5080

ER -

X-ray laser microscopy of rat sperm nuclei

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this