X-chromosome inactivation in monkey embryos and pluripotent stem cells

Masahito Tachibana, Hong Ma, Michelle L. Sparman, Hyo Sang Lee, Cathy M. Ramsey, Joy S. Woodward, Hathaitip Sritanaudomchai, Keith R. Masterson, Erin E. Wolff, Yibing Jia, Shoukhrat Mitalipov

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Inactivation of one X chromosome in female mammals (XX) compensates for the reduced dosage of X-linked gene expression in males (XY). However, the inner cell mass (ICM) of mouse preimplantation blastocysts and their in vitro counterparts, pluripotent embryonic stem cells (ESCs), initially maintain two active X chromosomes (XaXa). Random X chromosome inactivation (XCI) takes place in the ICM lineage after implantation or upon differentiation of ESCs, resulting in mosaic tissues composed of two cell types carrying either maternal or paternal active X chromosomes. While the status of XCI in human embryos and ICMs remains unknown, majority of human female ESCs show non-random XCI. We demonstrate here that rhesus monkey ESCs also display monoallelic expression and methylation of X-linked genes in agreement with non-random XCI. However, XIST and other X-linked genes were expressed from both chromosomes in isolated female monkey ICMs indicating that ex vivo pluripotent cells retain XaXa. Intriguingly, the trophectoderm (TE) in preimplantation monkey blastocysts also expressed X-linked genes from both alleles suggesting that, unlike the mouse, primate TE lineage does not support imprinted paternal XCI. Our results provide insights into the species-specific nature of XCI in the primate system and reveal fundamental epigenetic differences between in vitro and ex vivo primate pluripotent cells.

Original languageEnglish (US)
Pages (from-to)146-155
Number of pages10
JournalDevelopmental Biology
Volume371
Issue number2
DOIs
StatePublished - Nov 15 2012

Fingerprint

X Chromosome Inactivation
Pluripotent Stem Cells
Haplorhini
Embryonic Structures
X-Linked Genes
Embryonic Stem Cells
Primates
X Chromosome
Blastocyst
Cell Lineage
Macaca mulatta
Epigenomics
Methylation
Mammals
Chromosomes
Alleles
Mothers
Gene Expression

Keywords

  • Blastocyst
  • Embryonic stem cells
  • Inner cell mass
  • Primates
  • X-inactivation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Tachibana, M., Ma, H., Sparman, M. L., Lee, H. S., Ramsey, C. M., Woodward, J. S., ... Mitalipov, S. (2012). X-chromosome inactivation in monkey embryos and pluripotent stem cells. Developmental Biology, 371(2), 146-155. https://doi.org/10.1016/j.ydbio.2012.08.009

X-chromosome inactivation in monkey embryos and pluripotent stem cells. / Tachibana, Masahito; Ma, Hong; Sparman, Michelle L.; Lee, Hyo Sang; Ramsey, Cathy M.; Woodward, Joy S.; Sritanaudomchai, Hathaitip; Masterson, Keith R.; Wolff, Erin E.; Jia, Yibing; Mitalipov, Shoukhrat.

In: Developmental Biology, Vol. 371, No. 2, 15.11.2012, p. 146-155.

Research output: Contribution to journalArticle

Tachibana, M, Ma, H, Sparman, ML, Lee, HS, Ramsey, CM, Woodward, JS, Sritanaudomchai, H, Masterson, KR, Wolff, EE, Jia, Y & Mitalipov, S 2012, 'X-chromosome inactivation in monkey embryos and pluripotent stem cells', Developmental Biology, vol. 371, no. 2, pp. 146-155. https://doi.org/10.1016/j.ydbio.2012.08.009
Tachibana M, Ma H, Sparman ML, Lee HS, Ramsey CM, Woodward JS et al. X-chromosome inactivation in monkey embryos and pluripotent stem cells. Developmental Biology. 2012 Nov 15;371(2):146-155. https://doi.org/10.1016/j.ydbio.2012.08.009
Tachibana, Masahito ; Ma, Hong ; Sparman, Michelle L. ; Lee, Hyo Sang ; Ramsey, Cathy M. ; Woodward, Joy S. ; Sritanaudomchai, Hathaitip ; Masterson, Keith R. ; Wolff, Erin E. ; Jia, Yibing ; Mitalipov, Shoukhrat. / X-chromosome inactivation in monkey embryos and pluripotent stem cells. In: Developmental Biology. 2012 ; Vol. 371, No. 2. pp. 146-155.
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