Whole body nitrogen kinetics and their relationship to growth in short children treated with recombinant human growth hormone

David P. Dempsher, Dennis M. Bier, Sherida E. Tollefsen, Peter S. Rotwein, William H. Daughaday, Mary Catherine Jensen, John P. Galgani, Ellen Heath-Monnig, Bakula Trivedi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We studied the effects of growth hormone on retention of 15N-labeled amino acids in 34 short, prepubertal, growth hormone-sufficient children and three growth hormone-deficient subjects. All 34 non-growth hormone-deficient children had apparently normal circulating growth hormone molecules and no mutations were detected in the growth hormone or IGF-I genes of any subjects. Fibroblasts from 34 children responded normally when challenged with recombinant human IGF-I. During the last 72 h of a 4-d challenge with recombinant human growth hormone (16 μg/kg body wt), retention of a mixed 15N-amino acid dose varied between 5.7 and 50.5%. Whole body protein synthesis, breakdown, and net anabolism calculated from the 15N kinetics were all increased by the acute growth hormone challenge. However, no routine clinical feature or laboratory determination correlated with the nitrogen retention response. After subsequent treatment (75 μg/kg three times a week) with recombinant human growth hormone for 1 y, there was a significant increase in height velocity, but this increase was not related significantly to pretreatment variables other than inversely to pretreatment height velocity. There was a significant (p = 0.03) correlation between the change in height velocity Z score and the degree of nitrogen retention to acute challenge with growth hormone, but this correlation was too weak (r = 0.37) to be of practical value in predicting the treatment growth response in an individual child.

Original languageEnglish (US)
Pages (from-to)394-400
Number of pages7
JournalPediatric Research
Volume28
Issue number4
StatePublished - 1990
Externally publishedYes

Fingerprint

Human Growth Hormone
Growth Hormone
Nitrogen
Growth
Insulin-Like Growth Factor I
Amino Acids
Fibroblasts
Hormones
Mutation
Therapeutics

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Dempsher, D. P., Bier, D. M., Tollefsen, S. E., Rotwein, P. S., Daughaday, W. H., Jensen, M. C., ... Trivedi, B. (1990). Whole body nitrogen kinetics and their relationship to growth in short children treated with recombinant human growth hormone. Pediatric Research, 28(4), 394-400.

Whole body nitrogen kinetics and their relationship to growth in short children treated with recombinant human growth hormone. / Dempsher, David P.; Bier, Dennis M.; Tollefsen, Sherida E.; Rotwein, Peter S.; Daughaday, William H.; Jensen, Mary Catherine; Galgani, John P.; Heath-Monnig, Ellen; Trivedi, Bakula.

In: Pediatric Research, Vol. 28, No. 4, 1990, p. 394-400.

Research output: Contribution to journalArticle

Dempsher, DP, Bier, DM, Tollefsen, SE, Rotwein, PS, Daughaday, WH, Jensen, MC, Galgani, JP, Heath-Monnig, E & Trivedi, B 1990, 'Whole body nitrogen kinetics and their relationship to growth in short children treated with recombinant human growth hormone', Pediatric Research, vol. 28, no. 4, pp. 394-400.
Dempsher DP, Bier DM, Tollefsen SE, Rotwein PS, Daughaday WH, Jensen MC et al. Whole body nitrogen kinetics and their relationship to growth in short children treated with recombinant human growth hormone. Pediatric Research. 1990;28(4):394-400.
Dempsher, David P. ; Bier, Dennis M. ; Tollefsen, Sherida E. ; Rotwein, Peter S. ; Daughaday, William H. ; Jensen, Mary Catherine ; Galgani, John P. ; Heath-Monnig, Ellen ; Trivedi, Bakula. / Whole body nitrogen kinetics and their relationship to growth in short children treated with recombinant human growth hormone. In: Pediatric Research. 1990 ; Vol. 28, No. 4. pp. 394-400.
@article{e3563779e337435a946ee085b9ace00f,
title = "Whole body nitrogen kinetics and their relationship to growth in short children treated with recombinant human growth hormone",
abstract = "We studied the effects of growth hormone on retention of 15N-labeled amino acids in 34 short, prepubertal, growth hormone-sufficient children and three growth hormone-deficient subjects. All 34 non-growth hormone-deficient children had apparently normal circulating growth hormone molecules and no mutations were detected in the growth hormone or IGF-I genes of any subjects. Fibroblasts from 34 children responded normally when challenged with recombinant human IGF-I. During the last 72 h of a 4-d challenge with recombinant human growth hormone (16 μg/kg body wt), retention of a mixed 15N-amino acid dose varied between 5.7 and 50.5{\%}. Whole body protein synthesis, breakdown, and net anabolism calculated from the 15N kinetics were all increased by the acute growth hormone challenge. However, no routine clinical feature or laboratory determination correlated with the nitrogen retention response. After subsequent treatment (75 μg/kg three times a week) with recombinant human growth hormone for 1 y, there was a significant increase in height velocity, but this increase was not related significantly to pretreatment variables other than inversely to pretreatment height velocity. There was a significant (p = 0.03) correlation between the change in height velocity Z score and the degree of nitrogen retention to acute challenge with growth hormone, but this correlation was too weak (r = 0.37) to be of practical value in predicting the treatment growth response in an individual child.",
author = "Dempsher, {David P.} and Bier, {Dennis M.} and Tollefsen, {Sherida E.} and Rotwein, {Peter S.} and Daughaday, {William H.} and Jensen, {Mary Catherine} and Galgani, {John P.} and Ellen Heath-Monnig and Bakula Trivedi",
year = "1990",
language = "English (US)",
volume = "28",
pages = "394--400",
journal = "Pediatric Research",
issn = "0031-3998",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Whole body nitrogen kinetics and their relationship to growth in short children treated with recombinant human growth hormone

AU - Dempsher, David P.

AU - Bier, Dennis M.

AU - Tollefsen, Sherida E.

AU - Rotwein, Peter S.

AU - Daughaday, William H.

AU - Jensen, Mary Catherine

AU - Galgani, John P.

AU - Heath-Monnig, Ellen

AU - Trivedi, Bakula

PY - 1990

Y1 - 1990

N2 - We studied the effects of growth hormone on retention of 15N-labeled amino acids in 34 short, prepubertal, growth hormone-sufficient children and three growth hormone-deficient subjects. All 34 non-growth hormone-deficient children had apparently normal circulating growth hormone molecules and no mutations were detected in the growth hormone or IGF-I genes of any subjects. Fibroblasts from 34 children responded normally when challenged with recombinant human IGF-I. During the last 72 h of a 4-d challenge with recombinant human growth hormone (16 μg/kg body wt), retention of a mixed 15N-amino acid dose varied between 5.7 and 50.5%. Whole body protein synthesis, breakdown, and net anabolism calculated from the 15N kinetics were all increased by the acute growth hormone challenge. However, no routine clinical feature or laboratory determination correlated with the nitrogen retention response. After subsequent treatment (75 μg/kg three times a week) with recombinant human growth hormone for 1 y, there was a significant increase in height velocity, but this increase was not related significantly to pretreatment variables other than inversely to pretreatment height velocity. There was a significant (p = 0.03) correlation between the change in height velocity Z score and the degree of nitrogen retention to acute challenge with growth hormone, but this correlation was too weak (r = 0.37) to be of practical value in predicting the treatment growth response in an individual child.

AB - We studied the effects of growth hormone on retention of 15N-labeled amino acids in 34 short, prepubertal, growth hormone-sufficient children and three growth hormone-deficient subjects. All 34 non-growth hormone-deficient children had apparently normal circulating growth hormone molecules and no mutations were detected in the growth hormone or IGF-I genes of any subjects. Fibroblasts from 34 children responded normally when challenged with recombinant human IGF-I. During the last 72 h of a 4-d challenge with recombinant human growth hormone (16 μg/kg body wt), retention of a mixed 15N-amino acid dose varied between 5.7 and 50.5%. Whole body protein synthesis, breakdown, and net anabolism calculated from the 15N kinetics were all increased by the acute growth hormone challenge. However, no routine clinical feature or laboratory determination correlated with the nitrogen retention response. After subsequent treatment (75 μg/kg three times a week) with recombinant human growth hormone for 1 y, there was a significant increase in height velocity, but this increase was not related significantly to pretreatment variables other than inversely to pretreatment height velocity. There was a significant (p = 0.03) correlation between the change in height velocity Z score and the degree of nitrogen retention to acute challenge with growth hormone, but this correlation was too weak (r = 0.37) to be of practical value in predicting the treatment growth response in an individual child.

UR - http://www.scopus.com/inward/record.url?scp=0025102135&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025102135&partnerID=8YFLogxK

M3 - Article

VL - 28

SP - 394

EP - 400

JO - Pediatric Research

JF - Pediatric Research

SN - 0031-3998

IS - 4

ER -