TY - JOUR
T1 - What is normal? A characterization of the values and variability in reproductive endpoints of the fathead minnow, Pimephales promelas
AU - Watanabe, Karen H.
AU - Jensen, Kathleen M.
AU - Orlando, Edward F.
AU - Ankley, Gerald T.
N1 - Funding Information:
The authors gratefully acknowledge Ms. Zhenhong Li who assisted with calculating spawn intervals. This research was supported in part by a Cooperative Agreement to the University of Florida, FL (N. Denslow), Oregon Health & Science University, OR (KW), Purdue University, IN (M. Sepulveda) and Florida Atlantic University, FL (EO) from the US Environmental Protection Agency (EPA) Science to Achieve Results program (STAR; Assistance Agreement # RD-83184801-0). This research was also supported by resources from the National Center for Computational Toxicology of the EPA Office of Research and Development, awarded to the Ecological Exposure Research and Ecosystem Research Divisions (National Exposure Research Laboratory) in Cincinnati, OH and Athens, GA, respectively, and the Mid-Continent Ecology Division (National Health and Environmental Effects Research Laboratory) in Duluth, MN.
PY - 2007/9
Y1 - 2007/9
N2 - Jensen et al. [Jensen, K.M., Korte, J.J., Kahl, M.D., Pasha, M.S., Ankley, G.T., 2001. Aspects of basic reproductive biology and endocrinology in the fathead minnow (Pimephales promelas). Comp. Biochem. Physiol. C 128, 127-141.] investigated aspects of the normal reproductive biology of the fathead minnow (FHM, P. promelas), and subsequent studies have generated a large amount of additional reproductive data for endpoints such as plasma steroid hormone and vitellogenin concentrations, spawn interval, and secondary sex characteristics (i.e., nuptial tubercle score and fat pad weight). These data were analyzed and fitted with statistical distributions to improve understanding of the variability in normal, unexposed, adult male (n = 154) and female (n = 186) FHM. Summary statistics for most endpoints were consistent with results from other more limited studies of FHMs. Male fat pad weight, and in both sexes gonad and liver weights were found to be proportional to body weight. Multiple statistical distributions were found to characterize each endpoint with the exception of spawn interval. Based on one of the largest datasets ever compiled for controlled studies, results presented herein provide a robust point of reference for the quantitative assessment of reproductive processes in the fathead minnow.
AB - Jensen et al. [Jensen, K.M., Korte, J.J., Kahl, M.D., Pasha, M.S., Ankley, G.T., 2001. Aspects of basic reproductive biology and endocrinology in the fathead minnow (Pimephales promelas). Comp. Biochem. Physiol. C 128, 127-141.] investigated aspects of the normal reproductive biology of the fathead minnow (FHM, P. promelas), and subsequent studies have generated a large amount of additional reproductive data for endpoints such as plasma steroid hormone and vitellogenin concentrations, spawn interval, and secondary sex characteristics (i.e., nuptial tubercle score and fat pad weight). These data were analyzed and fitted with statistical distributions to improve understanding of the variability in normal, unexposed, adult male (n = 154) and female (n = 186) FHM. Summary statistics for most endpoints were consistent with results from other more limited studies of FHMs. Male fat pad weight, and in both sexes gonad and liver weights were found to be proportional to body weight. Multiple statistical distributions were found to characterize each endpoint with the exception of spawn interval. Based on one of the largest datasets ever compiled for controlled studies, results presented herein provide a robust point of reference for the quantitative assessment of reproductive processes in the fathead minnow.
KW - Biological variability
KW - Fathead minnow
KW - Reproduction
KW - Steroid hormones
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U2 - 10.1016/j.cbpc.2007.04.015
DO - 10.1016/j.cbpc.2007.04.015
M3 - Article
C2 - 17600770
AN - SCOPUS:34547552220
SN - 1532-0456
VL - 146
SP - 348
EP - 356
JO - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
IS - 3
ER -