Wavefront sensorless adaptive optics fluorescence biomicroscope for in vivo retinal imaging in mice

Daniel J. Wahl, Yifan Jian, Stefano Bonora, Robert J. Zawadzki, Marinko V. Sarunic

Research output: Contribution to journalArticle

28 Scopus citations


Cellular-resolution in vivo fluorescence imaging is a valuable tool for longitudinal studies of retinal function in vision research. Wavefront sensorless adaptive optics (WSAO) is a developing technology that enables high-resolution imaging of the mouse retina. In place of the conventional method of using a Shack-Hartmann wavefront sensor to measure the aberrations directly, WSAO uses an image quality metric and a search algorithm to drive the shape of the adaptive element (i.e. deformable mirror). WSAO is a robust approach to AO and it is compatible with a compact, low-cost lens-based system. In this report, we demonstrated a hill-climbing algorithm for WSAO with a variable focus lens and deformable mirror for non-invasive in vivo imaging of EGFP (enhanced green fluorescent protein) labelled ganglion cells and microglia cells in the mouse retina.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalBiomedical Optics Express
Issue number1
StatePublished - Dec 4 2015
Externally publishedYes



  • Active or adaptive optics
  • Imaging systems
  • Ophthalmic optics and devices
  • Ophthalmology

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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