Wavefront sensorless adaptive optics fluorescence biomicroscope for in vivo retinal imaging in mice

Daniel J. Wahl, Yifan Jian, Stefano Bonora, Robert J. Zawadzki, Marinko V. Sarunic

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Cellular-resolution in vivo fluorescence imaging is a valuable tool for longitudinal studies of retinal function in vision research. Wavefront sensorless adaptive optics (WSAO) is a developing technology that enables high-resolution imaging of the mouse retina. In place of the conventional method of using a Shack-Hartmann wavefront sensor to measure the aberrations directly, WSAO uses an image quality metric and a search algorithm to drive the shape of the adaptive element (i.e. deformable mirror). WSAO is a robust approach to AO and it is compatible with a compact, low-cost lens-based system. In this report, we demonstrated a hill-climbing algorithm for WSAO with a variable focus lens and deformable mirror for non-invasive in vivo imaging of EGFP (enhanced green fluorescent protein) labelled ganglion cells and microglia cells in the mouse retina.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalBiomedical Optics Express
Volume7
Issue number1
DOIs
StatePublished - Dec 4 2015
Externally publishedYes

Fingerprint

adaptive optics
Lenses
mice
Retina
Fluorescence
fluorescence
retina
deformable mirrors
Optical Imaging
Microglia
Ganglia
Longitudinal Studies
lenses
Technology
Costs and Cost Analysis
cells
Research
aberration
mirrors
proteins

Keywords

  • Active or adaptive optics
  • Imaging systems
  • Ophthalmic optics and devices
  • Ophthalmology

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Wavefront sensorless adaptive optics fluorescence biomicroscope for in vivo retinal imaging in mice. / Wahl, Daniel J.; Jian, Yifan; Bonora, Stefano; Zawadzki, Robert J.; Sarunic, Marinko V.

In: Biomedical Optics Express, Vol. 7, No. 1, 04.12.2015, p. 1-12.

Research output: Contribution to journalArticle

Wahl, Daniel J. ; Jian, Yifan ; Bonora, Stefano ; Zawadzki, Robert J. ; Sarunic, Marinko V. / Wavefront sensorless adaptive optics fluorescence biomicroscope for in vivo retinal imaging in mice. In: Biomedical Optics Express. 2015 ; Vol. 7, No. 1. pp. 1-12.
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