Abstract
Cellular-resolution in vivo fluorescence imaging is a valuable tool for longitudinal studies of retinal function in vision research. Wavefront sensorless adaptive optics (WSAO) is a developing technology that enables high-resolution imaging of the mouse retina. In place of the conventional method of using a Shack-Hartmann wavefront sensor to measure the aberrations directly, WSAO uses an image quality metric and a search algorithm to drive the shape of the adaptive element (i.e. deformable mirror). WSAO is a robust approach to AO and it is compatible with a compact, low-cost lens-based system. In this report, we demonstrated a hill-climbing algorithm for WSAO with a variable focus lens and deformable mirror for non-invasive in vivo imaging of EGFP (enhanced green fluorescent protein) labelled ganglion cells and microglia cells in the mouse retina.
Original language | English (US) |
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Pages (from-to) | 1-12 |
Number of pages | 12 |
Journal | Biomedical Optics Express |
Volume | 7 |
Issue number | 1 |
DOIs | |
State | Published - Dec 4 2015 |
Externally published | Yes |
Keywords
- Active or adaptive optics
- Imaging systems
- Ophthalmic optics and devices
- Ophthalmology
ASJC Scopus subject areas
- Biotechnology
- Atomic and Molecular Physics, and Optics