Abstract
Experiments were performed in rats to test the hypothesis that adrenal mRNA levels of tyrosine hydroxylase (TH) and the norepinephrine transporter (NET) would be modified by water deprivation via activation of the sympathetic nervous system. TH and NET mRNA levels were measured using the ribonuclease protection assay. Adrenal TH mRNA was higher (P <0.001) in water-deprived (921 ± 39 fg/μg total RNA) compared with the water-replete rats (657 ± 45 fg/μg total RNA). In contrast, water deprivation decreased (P <0.01) adrenal NET mRNA levels (275 ± 66 vs. 433 ± 63 fg/μg total RNA). The dehydration-induced increase in TH mRNA was prevented by prior splanchnicectomy, but the decrease in NET mRNA was produced even in the absence of adrenal nerves. Water deprivation also increased (P <0.05) plasma adrenocorticotropic hormone (84 ± 16 vs. 42 ± 14 pg/ml) and corticosterone (358 ± 87 vs. 44 ± 15 ng/ml) levels. Interestingly, the corticosterone response was reduced (P <0.05) by unilateral adrenal denervation. These results suggest that water deprivation increases both adrenal medullary and adrenocortical activity at least in part by stimulation of sympathetic nerve activity.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Regulatory Integrative and Comparative Physiology |
| Volume | 272 |
| Issue number | 6 41-6 |
| State | Published - 1997 |
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Keywords
- Adrenocorticotropic hormone
- Angiotensin II
- Corticosterone
- Dehydration
- Sympathetic nervous system
ASJC Scopus subject areas
- Physiology
- Physiology (medical)
Cite this
Water deprivation and rat adrenal mRNAs for tyrosine hydroxylase and the norepinephrine transporter. / Brooks, Virginia; Huhtala, Timothy A.; Silliman, Terri L.; Engeland, William C.
In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 272, No. 6 41-6, 1997.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Water deprivation and rat adrenal mRNAs for tyrosine hydroxylase and the norepinephrine transporter
AU - Brooks, Virginia
AU - Huhtala, Timothy A.
AU - Silliman, Terri L.
AU - Engeland, William C.
PY - 1997
Y1 - 1997
N2 - Experiments were performed in rats to test the hypothesis that adrenal mRNA levels of tyrosine hydroxylase (TH) and the norepinephrine transporter (NET) would be modified by water deprivation via activation of the sympathetic nervous system. TH and NET mRNA levels were measured using the ribonuclease protection assay. Adrenal TH mRNA was higher (P <0.001) in water-deprived (921 ± 39 fg/μg total RNA) compared with the water-replete rats (657 ± 45 fg/μg total RNA). In contrast, water deprivation decreased (P <0.01) adrenal NET mRNA levels (275 ± 66 vs. 433 ± 63 fg/μg total RNA). The dehydration-induced increase in TH mRNA was prevented by prior splanchnicectomy, but the decrease in NET mRNA was produced even in the absence of adrenal nerves. Water deprivation also increased (P <0.05) plasma adrenocorticotropic hormone (84 ± 16 vs. 42 ± 14 pg/ml) and corticosterone (358 ± 87 vs. 44 ± 15 ng/ml) levels. Interestingly, the corticosterone response was reduced (P <0.05) by unilateral adrenal denervation. These results suggest that water deprivation increases both adrenal medullary and adrenocortical activity at least in part by stimulation of sympathetic nerve activity.
AB - Experiments were performed in rats to test the hypothesis that adrenal mRNA levels of tyrosine hydroxylase (TH) and the norepinephrine transporter (NET) would be modified by water deprivation via activation of the sympathetic nervous system. TH and NET mRNA levels were measured using the ribonuclease protection assay. Adrenal TH mRNA was higher (P <0.001) in water-deprived (921 ± 39 fg/μg total RNA) compared with the water-replete rats (657 ± 45 fg/μg total RNA). In contrast, water deprivation decreased (P <0.01) adrenal NET mRNA levels (275 ± 66 vs. 433 ± 63 fg/μg total RNA). The dehydration-induced increase in TH mRNA was prevented by prior splanchnicectomy, but the decrease in NET mRNA was produced even in the absence of adrenal nerves. Water deprivation also increased (P <0.05) plasma adrenocorticotropic hormone (84 ± 16 vs. 42 ± 14 pg/ml) and corticosterone (358 ± 87 vs. 44 ± 15 ng/ml) levels. Interestingly, the corticosterone response was reduced (P <0.05) by unilateral adrenal denervation. These results suggest that water deprivation increases both adrenal medullary and adrenocortical activity at least in part by stimulation of sympathetic nerve activity.
KW - Adrenocorticotropic hormone
KW - Angiotensin II
KW - Corticosterone
KW - Dehydration
KW - Sympathetic nervous system
UR - http://www.scopus.com/inward/record.url?scp=0030789655&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030789655&partnerID=8YFLogxK
M3 - Article
C2 - 9227605
AN - SCOPUS:0030789655
VL - 272
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 6 41-6
ER -